Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osmoregulation in Fishes02:32

Osmoregulation in Fishes

49.3K
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
49.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel adomavirus from proliferative skin lesions of a broadnose sevengill shark (Notorynchus cepedianus).

Npj viruses·2026
Same author

A Single Cell Atlas of the Newt Iris During Lens Regeneration.

bioRxiv : the preprint server for biology·2026
Same author

Ceftriaxone to Prevent Early-Onset Pneumonia in Comatose Patients Following Out-of-Hospital Cardiac Arrest: A Pilot Randomized Controlled Trial and Resistome Assessment (PROTECT).

Chest·2025
Same author

Protein arginine methyltransferase 5 sustains Tip60-EP400 complex via SRSF1 in Merkel cell carcinoma.

Life science alliance·2025
Same author

What is eDNA method standardisation and why do we need it?

Metabarcoding and metagenomics·2025
Same author

An Enigmatic Wild Passerine Mortality Event in the Eastern United States.

Veterinary sciences·2025

Related Experiment Video

Updated: Jun 3, 2025

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish
10:56

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Published on: March 6, 2014

12.5K

An optimized eDNA protocol for fish tracking in estuarine environments.

Fouad El Baidouri1, Alison W Watts2, Jeffrey T Miller3

  • 1Department of Civil & Environmental Engineering, University of New Hampshire, Durham, NH, 03824, USA. fouad.elbaidouri@unh.edu.

Scientific Reports
|January 8, 2025
PubMed
Summary

Environmental DNA (eDNA) analysis can now detect more fish species in complex estuarine systems. This improved method overcomes PCR inhibition and increases species recovery for better biodiversity monitoring.

Keywords:
EstuarineFish speciesMiFishMultiplex PCRPCR inhibitionPlatinum SuperFi IIeDNA

More Related Videos

Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster Nephrops norvegicus
05:57

Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster Nephrops norvegicus

Published on: April 8, 2019

6.8K
Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
14:03

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

11.0K

Related Experiment Videos

Last Updated: Jun 3, 2025

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish
10:56

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

Published on: March 6, 2014

12.5K
Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster Nephrops norvegicus
05:57

Long-term Video Tracking of Cohoused Aquatic Animals: A Case Study of the Daily Locomotor Activity of the Norway Lobster Nephrops norvegicus

Published on: April 8, 2019

6.8K
Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
14:03

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

11.0K

Area of Science:

  • Environmental DNA (eDNA) analysis
  • Aquatic biodiversity assessment
  • Estuarine ecosystem monitoring

Background:

  • Environmental DNA (eDNA) is a powerful, noninvasive tool for biodiversity research.
  • Estuarine systems present unique challenges for eDNA, including PCR inhibition and low fish DNA recovery.
  • Existing eDNA methods struggle to accurately assess fish communities in turbid, productive estuarine environments.

Purpose of the Study:

  • To develop and present an optimized approach for detecting fish eDNA in challenging estuarine systems.
  • To improve the accuracy and efficiency of fish biodiversity assessments in complex aquatic environments.

Main Methods:

  • A hybrid eDNA workflow was implemented, combining bead-based extraction with inhibition removal.
  • High-fidelity DNA polymerase (Platinum SuperFi II) and multiplexed universal MiFish primers were utilized.
  • The method was tested on diverse estuarine samples known to cause PCR inhibition.

Main Results:

  • The optimized eDNA approach more than doubled the number of recovered fish species compared to standard methods.
  • Significant reduction in off-target amplification was observed, improving data reliability.
  • Successful detection of fish species in previously challenging, inhibited estuarine samples.

Conclusions:

  • This enhanced eDNA workflow effectively overcomes limitations in analyzing estuarine samples.
  • The method provides a more comprehensive and reliable assessment of fish biodiversity in complex aquatic ecosystems.
  • This approach advances the application of eDNA for effective environmental monitoring and conservation.