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UF-5000 urinary erythrocyte parameters versus urinary aberrant erythrocytes and acanthocytes for diagnosing IgA
Shuangshuang Lv1, Yi Fang2, Xiaxuan Hu2
1Clinical Laboratory, Dongyang People's Hospital, No. 60 Wuning West Road, Dongyang City, 322100, Zhejiang, China. 15958490336@139.com.
This study evaluated the diagnostic value of the automated UF-5000 parameters and compared it with that of aberrant erythrocytes and acanthocytes classified by microscopy for identifying IgA glomerular hematuria to propose a predictive model for clinical use. It also compared correlations between erythrocyte parameters and malformed erythrocytes. Urine samples from 53 biopsy-proven IgA hematuria cases and 143 non-IGA nephropathic hematuria cases as controls were analyzed. The ratio of small red blood cells to nonlysed red blood cells (UF-sRBC%) and lysed red blood cells (lysed RBCs) showed good diagnostic performance for IgA glomerular hematuria (area under the curve [AUC] = 0.857 [P = 0.000] and AUC = 0.860 [P = 0.000], respectively). Combining UF-sRBC%, lysed RBCs, and urine protein dry chemistry improved the diagnostic accuracy (AUC = 0.967; positive predictive value [PPV] = 91.89%; negative predictive value [NPV] = 93.10%; P = 0.000). This approach surpassed traditional microscopy for aberrant erythrocytes (AUC = 0.895; PPV = 62.27%; NPV = 88.66%; P = 0.008) and acanthocytes (AUC = 0.868; PPV = 72.97%; NPV = 83.65%; P = 0.006). The erythrocyte size index was negatively correlated with the proportion of urinary aberrant erythrocytes (r = - 0.787; P = 0.000). The UF-5000 erythrocyte parameters facilitate rapid identification of IgA nephropathy and could replace manual microscopy.
This study evaluated the diagnostic value of the automated UF-5000 parameters and compared it with that of aberrant erythrocytes and acanthocytes classified by microscopy for identifying IgA glomerular hematuria to propose a predictive model for clinical use. It also compared correlations between erythrocyte parameters and malformed erythrocytes. Urine samples from 53 biopsy-proven IgA hematuria cases and 143 non-IGA nephropathic hematuria cases as controls were analyzed. The ratio of small red blood cells to nonlysed red blood cells (UF-sRBC%) and lysed red blood cells (lysed RBCs) showed good diagnostic performance for IgA glomerular hematuria (area under the curve [AUC] = 0.857 [P = 0.000] and AUC = 0.860 [P = 0.000], respectively). Combining UF-sRBC%, lysed RBCs, and urine protein dry chemistry improved the diagnostic accuracy (AUC = 0.967; positive predictive value [PPV] = 91.89%; negative predictive value [NPV] = 93.10%; P = 0.000). This approach surpassed traditional microscopy for aberrant erythrocytes (AUC = 0.895; PPV = 62.27%; NPV = 88.66%; P = 0.008) and acanthocytes (AUC = 0.868; PPV = 72.97%; NPV = 83.65%; P = 0.006). The erythrocyte size index was negatively correlated with the proportion of urinary aberrant erythrocytes (r = - 0.787; P = 0.000). The UF-5000 erythrocyte parameters facilitate rapid identification of IgA nephropathy and could replace manual microscopy.
