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Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy
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Elucidating the anticancer potential of dendrobine in renal cell carcinoma treatment

Xing Jia1, Zixuan Chen1, Xingyu Chen2

  • 1Department of Urology, Tongren Hospital Shanghai Jiao Tong University School of Medicine, No.1111 Xian Xia Road, Shanghai, 200336, China.

Naunyn-Schmiedeberg'S Archives of Pharmacology
|January 8, 2025
PubMed

Insights

Dendrobine, a natural compound, shows promise in treating renal cell carcinoma (RCC) by inhibiting cancer cell growth, migration, and inducing apoptosis. This study identifies STAT3 as a key target and reveals its mechanism via the PI3K/Akt pathway.

Area of Science:

  • Oncology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Renal cell carcinoma (RCC) remains a significant health concern, with drug resistance and adverse effects limiting current treatments.
  • Natural products offer a promising avenue for novel cancer therapeutics, yet their specific anti-RCC mechanisms are often underexplored.

Purpose of the Study:

  • To investigate the therapeutic potential and underlying mechanisms of Dendrobine, a natural product, against renal cell carcinoma (RCC).
  • To identify key molecular targets and signaling pathways involved in Dendrobine's anti-RCC effects using network pharmacology and in vitro validation.

Main Methods:

  • Network pharmacology was employed to predict Dendrobine's targets in RCC, identifying STAT3 as a crucial node.
  • In vitro assays including CCK-8, clonogenic formation, EdU staining, wound healing, invasion assays, and Hoechst 33342/PI co-staining were performed.
  • Western blot analysis was used to investigate the impact of Dendrobine on the PI3K/Akt signaling pathway.

Main Results:

  • Dendrobine significantly inhibited RCC cell viability, proliferation, migration, and invasion in a dose-dependent manner.
  • Dendrobine was confirmed to induce apoptosis in RCC cells.
  • Mechanistic studies revealed that Dendrobine targets the PI3K/Akt signaling pathway by inhibiting p-PI3K, p-Akt, and p-Erk expression.

Conclusions:

  • Dendrobine exhibits potent anti-RCC activity through multiple mechanisms, including the inhibition of cell growth and induction of apoptosis.
  • The PI3K/Akt signaling pathway is a key mediator of Dendrobine's anti-cancer effects in RCC.
  • Dendrobine represents a potential therapeutic candidate for renal cell carcinoma, warranting further investigation.
Keywords:
DendrobineNatural productsNetwork pharmacologyPI3K/AktRenal cell carcinoma

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