Insect Cytochrome P450 Database: An Integrated Resource of Genetic Diversity, Evolution and Function

Area of Science:

• * Entomology
• * Bioinformatics
• * Molecular Biology

Background:

• * Insects are diverse and interact with humans via resources, disease, and agriculture.
• * Cytochrome P450 monooxygenases (P450s) are vital insect proteins involved in metabolism and detoxification.
• * Understanding insect P450s is key for pest control and insecticide development.

Purpose of the Study:

• * To establish an integrated bioinformatics platform for insect P450s.
• * To facilitate research on the evolution and function of insect P450s.
• * To support the development and application of novel insecticides.

Main Methods:

• * Compiled 66,477 P450s from public databases and 682 insect genomes.
• * Utilized a standardized bioinformatics protocol for P450 collection and analysis.
• * Predicted protein structures using ESMFold and visualized with WeView.
• * Developed web services including BLAST, homology modeling, and molecular docking.

Main Results:

• * Created the Insect Cytochrome P450 database (ICPD) with extensive P450 data.
• * Constructed phylogenetic relationships for P450 genes across species.
• * Provided tools for analyzing P450 protein structures and catalytic activities.

Conclusions:

• * ICPD offers a valuable resource for systematic investigation of insect P450s.
• * The database will advance understanding of P450 evolution and function.
• * ICPD serves as a powerful tool for guiding insecticide design and application.