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Investigating Intestinal Inflammation in DSS-induced Model of IBD
Published on: February 1, 2012
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Alterations in Ileal Secretory Cells of The DSS-Induced Colitis Model Mice
Kenta Nakamura1,2, Ryoko Baba2, Keiji Kokubu2
1Third Department of Internal Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807-8555, Japan.
Acta Histochemica Et Cytochemica
|January 8, 2025
Summary
Dextran sodium sulphate (DSS) induced colitis alters ileal epithelial cells, particularly secretory cells, without affecting barrier function. These changes in the small intestine during inflammation offer new insights into inflammatory bowel disease.
Area of Science:
- Gastroenterology
- Immunology
- Cell Biology
Background:
- Inflammatory bowel disease (IBD) pathogenesis involves epithelial barrier dysfunction and immune responses.
- The dextran sodium sulphate (DSS)-induced colitis model primarily affects the colon, with limited understanding of its impact on the ileum.
- Investigating ileal changes is crucial for a comprehensive understanding of IBD.
Purpose of the Study:
- To histologically analyze the inflammatory and recovery phases in the ileum of DSS-induced colitis model mice.
- To evaluate the impact of DSS treatment on ileal epithelial cell morphology and function.
- To assess the integrity of the ileal barrier function using FITC-dextran.
Main Methods:
- Histological analysis of ileal tissues during inflammatory and recovery phases.
- Assessment of epithelial cell morphology, including crypts, goblet cells, and Paneth cells.
- Investigation of barrier function using 40 kDa FITC-dextran permeability assay.
- In situ hybridization and lectin histochemistry to analyze gene expression and glycosylation patterns.
Main Results:
- No significant histological damage was observed in the ileum during the inflammatory phase.
- Alterations included expanded crypts, hypertrophic goblet and Paneth cells, and increased mucus production.
- Ileal barrier function remained intact, with no FITC-dextran diffusion via the paracellular pathway.
- Differences in intestinal stem cell marker expression and secretory cell differentiation factors were noted.
- A decrease in FITC-positive Paneth cells was observed in the DSS group.
Conclusions:
- DSS-induced colitis induces significant morphological and functional alterations in ileal epithelial cells, especially secretory cells.
- Despite these cellular changes, the ileal barrier function remains intact in this model.
- Findings highlight the specific impact on ileal secretory cells, contributing to IBD pathogenesis understanding.
