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Updated: May 1, 2026
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
TtrAgo-mediated nucleic acid detection system and portable device for rapid detection of sexually transmitted
Jiexiu Zhang1, Zhongjing Su1, Qiulan Luo2
1Department of Histology and Embryology, Shantou University Medical College, Shantou, China.
Abstract:
The development of rapid and multiplexed point-of-care (POC) diagnostic tools is vital for the prevention and control of sexually transmitted diseases (STIs). Here, we developed a POC-comprehensive Thermococcus thioreducensArgonaute (TtrAgo)-mediated nucleic acid detection system (POC-CANDY) and palm-sized portable detection device "Owl-1" for the simultaneous detection of Ureaplasma urealyticum, Chlamydia trachomatis, Neisseria gonorrhoeae, human papillomavirus types 16/18 and antibiotic resistance molecular markers [tetM, and gyrA mutation (S91F)]. Using recombinase polymerase amplification (RPA), the optimized POC-CANDY could finish the whole detection procedure within 55 min and achieve a limit of detection of 10 copies/μL. When validated by clinical STI samples, POC-CANDY showed 100% consistency with quantitative PCR. Additionally, compared with the PfAgo-based system, POC-CANDY significantly improved the sensitivity of distinguishing single nucleotide variations. The results demonstrated that POC-CANDY can be easily applied locally or on site. This study also promotes the utility of the TtrAgo-mediated technique in clinical diagnosis.
