Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement-subcomponent-C1-inhibitor synthesis by human monocytes.

A C Yeung Laiwah, L Jones, A O Hamilton

    The Biochemical Journal
    |February 15, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Revolutionizing male contraception: Personal lubricants as a novel way to deliver the Human Contraception Antibody.

    bioRxiv : the preprint server for biology·2025
    Same author

    Prevention and treatment of Clostridium perfringens epsilon toxin intoxication in mice with a neutralizing monoclonal antibody (c4D7) produced in Nicotiana benthamiana.

    Toxicon : official journal of the International Society on Toxinology·2014
    Same author

    Fatal subaxial dislocation of cervical spine in rheumatoid arthritis.

    British medical journal·2010
    Same author

    Pregnancy-associated changes in peripheral blood lymphocyte subpopulations in normal Kuwaiti women.

    Gynecologic and obstetric investigation·2001
    Same author

    Central hypothyroidism associated with retinoid X receptor-selective ligands.

    The New England journal of medicine·1999
    Same author

    Thymoma-associated cutaneous graft-versus-host-like reaction.

    Clinical and experimental dermatology·1998
    Same journal

    Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

    The Biochemical journal·2026
    Same journal

    Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

    The Biochemical journal·2026
    Same journal

    Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

    The Biochemical journal·2026
    Same journal

    Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

    The Biochemical journal·2026
    Same journal

    Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

    The Biochemical journal·2026
    Same journal

    A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

    The Biochemical journal·2026
    See all related articles

    Human monocytes produce C1-inhibitor, a key immune protein. Studies suggest monocytes also secrete a distinct, uncharacterized molecule that inhibits C1 activity.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • C1-inhibitor (C1-INH) is a crucial regulator of the complement system.
    • Monocytes are key immune cells involved in inflammatory and regulatory processes.

    Purpose of the Study:

    • To investigate the production and function of C1-inhibitor by human monocytes.
    • To identify potential novel regulators of complement activity secreted by monocytes.

    Main Methods:

    • Radioimmunoassay was used to quantify C1-inhibitor in monocyte culture supernatants.
    • Cycloheximide was employed to assess protein synthesis inhibition.
    • Immunoprecipitation and SDS-PAGE were used to identify and characterize C1-inhibitor and its precursor.
    • Functional hemolytic assays were performed to assess C1-inhibitor activity.

    Related Experiment Videos

    Main Results:

    • Human monocytes synthesize and secrete functional C1-inhibitor.
    • C1-inhibitor production by monocytes is reversible and sensitive to cycloheximide.
    • A precursor form of C1-inhibitor (Mr 83,000) was identified intracellularly, with mature secreted C1-inhibitor (Mr 105,000) detected in supernatants.
    • Despite significant reduction in C1-inhibitor levels with cycloheximide, functional C1 activity was only partially inhibited, suggesting the presence of another inhibitory factor.

    Conclusions:

    • Human monocytes are a source of functional C1-inhibitor.
    • Monocytes secrete a novel, functionally active molecule that contributes to C1 inhibition, distinct from classical C1-inhibitor.