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Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

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Related Experiment Video

Updated: Jun 3, 2025

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Author Spotlight: High-Throughput In Vivo Leaf Inoculation for Accelerating Disease Resistance Screening in Poplar Hybrid Breeding

Published on: September 20, 2024

Poplar transformation with variable explant sources to maximize transformation efficiency.

Haiwei Lu^1,2, Sara Jawdy¹, Jin-Gui Chen^1,3

¹Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.

Scientific Reports

|January 8, 2025

Summary

Agrobacterium tumefaciens-mediated plant transformation is crucial for synthetic biology. This study shows root explants, like above-ground tissues, are effective for Populus regeneration and transformation, improving efficiency.

Keywords:

Agrobacterium tumefaciens Bioenergy Leaf Molecular factors Root Transformation

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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Last Updated: Jun 3, 2025

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Area of Science:

Plant Biotechnology
Synthetic Biology
Woody Perennial Crops

Background:

Agrobacterium tumefaciens-mediated plant transformation is vital for plant biology and synthetic biology applications.
Challenges remain in tissue culture recalcitrance and low transformation efficiency, particularly in crops.
Synthetic biology demands faster plant genetic manipulation cycles.

Purpose of the Study:

To investigate regeneration and transformation efficiency using diverse explants from Populus, a woody perennial bioenergy crop.
To compare the utility of above-ground (leaf, stem, petiole) and below-ground (root) explants.
To identify candidate genes associated with enhanced regeneration and transformation.

Main Methods:

Explants (leaf, stem, petiole, root) from Populus were used for regeneration and Agrobacterium tumefaciens-mediated transformation.
Regenerated and transformed plantlets were analyzed for morphology, reporter gene expression, and transcriptome profiles.
Transcriptome analysis was performed on explants during regeneration and transformation stages.

Main Results:

Root explants demonstrated significant regeneration capacity and susceptibility to Agrobacterium tumefaciens transformation.
Transformants derived from root explants showed comparable morphology, gene expression, and transcriptome profiles to those from above-ground tissues.
Transcriptome data revealed auxin/cytokinin signaling and developmental gene expression during organogenesis in leaf and root explants.
Candidate genes potentially linked to higher regeneration and transformation efficiencies were identified.

Conclusions:

Both above- and below-ground Populus organs are suitable for genetic transformation and tissue culture regeneration.
Utilizing diverse explants can maximize plant material use and enhance transformation productivity.
Transcriptome data provides insights into optimizing plant transformation efficiency.