Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Neurulation

Neurulation

Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Logic of Thalamic Inputs onto the Molecular Taxonomy of Cortical Neurons Reveals a Visual Hierarchy.

bioRxiv : the preprint server for biology·2026

Same author

Spatial and Molecular Progression of Neural Progenitor Cells in the Developing Human Dentate Gyrus.

bioRxiv : the preprint server for biology·2026

Same author

Cerebrovascular vulnerability and fibrosis in human brain aneurysms.

Nature neuroscience·2026

Same author

DISC1 mutant macaques capture behavioral and neural hallmarks of psychiatric disease.

National science review·2026

Same author

Embryonic MGE Precursor Cells Grafted into Adult Rat Striatum Integrate and Ameliorate Motor Symptoms in 6-OHDA-Lesioned Rats.

Cell stem cell·2026

Same author

Single-cell spatiotemporal dissection of the human maternal-fetal interface.

Nature·2026

Same journal

Keep the Hubble and James Webb Space Telescopes alive - the science is worth the price tag.

Nature·2026

Same journal

Say hello to hard helium.

Nature·2026

Same journal

How to avoid dementia - what the science really says.

Nature·2026

Same journal

Save Hubble: the race to preserve the space telescope kicks off.

Nature·2026

Same journal

How long can humans live? All evidence points to a maximum of 125 years.

Nature·2026

Same journal

Listen to Gen Z when it comes to AI in education.

Nature·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 3, 2025

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

Molecular and cellular dynamics of the developing human neocortex.

Li Wang^1,2, Cheng Wang^3,4, Juan A Moriano^3,4,5

¹The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, USA. Li.Wang@ucsf.edu.

|January 8, 2025

Summary

This summary is machine-generated.

This study maps human neocortex development, revealing gene regulatory networks and a tripotential progenitor cell type. Findings link these cells to glioblastoma and autism spectrum disorder risk.

More Related Videos

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Published on: April 3, 2013

Related Experiment Videos

Last Updated: Jun 3, 2025

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function

Published on: November 29, 2024

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Ex utero Electroporation and Whole Hemisphere Explants: A Simple Experimental Method for Studies of Early Cortical Development

Published on: April 3, 2013

Area of Science:

Neuroscience
Developmental Biology
Genomics

Background:

Human neocortex development involves complex gene regulation and cellular dynamics.
Understanding these processes is crucial for deciphering neural differentiation and associated disorders.

Purpose of the Study:

To create a comprehensive atlas of gene regulatory networks in the developing human neocortex.
To identify progenitor cell types and their lineage relationships during neurogenesis and gliogenesis.
To investigate the link between developmental processes and neurological diseases like glioblastoma and autism spectrum disorder.

Main Methods:

Paired single-nucleus chromatin accessibility and transcriptome sequencing from 38 human neocortical samples (first trimester to adolescence).
Spatial transcriptomic analysis for cellular organization and communication.
Progenitor purification and lineage-tracing experiments.
Integration with genome-wide association study data.

Main Results:

Catalogued cell-type, age, and area-specific gene regulatory networks.
Identified tripotential intermediate progenitor cells (Tri-IPCs) responsible for producing neurons, oligodendrocytes, and astrocytes.
Observed transcriptomic similarity between glioblastoma cells and Tri-IPCs.
Generated a disease-risk map linking autism spectrum disorder risk to second-trimester neurons.

Conclusions:

The developed atlas provides insights into the molecular and cellular dynamics of human neocortex development.
Tripotential intermediate progenitor cells play a key role in neural and glial cell production and may be hijacked in glioblastoma.
Specific developmental trajectories in the neocortex are associated with neurological disease risk, such as autism spectrum disorder.