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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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  11. Migration Of Primordial Germ Cells And Their Relationship Of Pgcs With Sex Development In Transgenic Germline-specific Fluorescent Freshwater Angelfish (pterophyllum Scalare).
  1. Home
  3. Research Domains
  5. Indigenous Studies
  7. Pacific Peoples Environmental Knowledges
  9. Pacific Peoples Fisheries And Customary Fisheries
  11. Migration Of Primordial Germ Cells And Their Relationship Of Pgcs With Sex Development In Transgenic Germline-specific Fluorescent Freshwater Angelfish (pterophyllum Scalare).

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Migration of primordial germ cells and their relationship of PGCs with sex development in transgenic germline-specific fluorescent freshwater angelfish (Pterophyllum scalare).

Wai-Kwan Chu1,2, Shih-Chin Huang1,3, Ching-Fong Chang1,2

  • 1Marine Molecular Genetics & Biotechnology Laboratory, Department of Aquaculture, National Taiwan Ocean University, Keelung, 202301, Taiwan.

Scientific Reports
|January 8, 2025

View abstract on PubMed

Summary
This summary is machine-generated.
Keywords:
Freshwater angelfishPGCs migrationSex determinationTransgenic

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Primordial germ cells (PGCs) in angelfish exhibit complex migration patterns and extended localization. PGCs abundance may influence sex determination, with fewer PGCs leading to males and more PGCs leading to females.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Ichthyology

Background:

  • Primordial germ cells (PGCs) are crucial for teleost reproduction, but their migration and localization vary across species.
  • Understanding PGCs dynamics is key to comprehending reproductive strategies in teleosts.

Purpose of the Study:

  • To develop a novel transgenic line for tracking PGCs in freshwater angelfish (Pterophyllum scalare).
  • To investigate PGCs migration, localization, and their potential role in sex determination in angelfish.

Main Methods:

  • Creation of a transgenic angelfish line (Tg(ddx4:TcCFP13-nanos3)) for fluorescently labeling PGCs.
  • In vivo observation and quantification of PGCs during early development.
  • Correlation analysis between PGCs abundance and subsequent sexual maturation.

Main Results:

  • A novel transgenic line successfully labeled PGCs in angelfish, revealing a complex, multistage migration.
  • Angelfish PGCs showed an extended localization period (168 hpf) compared to zebrafish (24 hpf).
  • A significant correlation was observed between PGCs abundance and sex determination: PGCs-reduced individuals predominantly became males (90%), while PGCs-rich individuals predominantly became females (83%).

Conclusions:

  • This study establishes a foundational understanding of PGCs migration and sex development in freshwater angelfish.
  • The developed in vivo PGCs tracking system offers a versatile tool for germ cell biology research in Cichlids and ornamental fish.
  • Findings provide valuable insights into teleost reproductive biology and the potential influence of PGCs on sex determination.