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Updated: Jun 3, 2025

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Published on: November 10, 2016
Pharmacokinetic analysis using single dilution assays: enhancing precision, reducing errors and increasing throughput
Saloumeh K Fischer1, Xiaome Xu2, Hayeun Ji2
1Bioanalytical Sciences (BAS), Genentech Research and Early Development, South San Francisco, CA, USA.
Background:
Technologies such as ELISA, MSD, and Gyrolab have been employed for quantifying protein therapeutics in clinical trials. However, these technologies have limitations with dynamic range often requiring multiple dilution steps, introducing potential errors and variability.
Results/Methodology:
A pharmacokinetics assay was successfully developed on the NUcleic acid Linked Immuno-Sandwich Assay (NULISA) platform with a concentration dynamic range exceeding 6 logs. This enabled assessment of all clinical samples across different concentrations with a single dilution, yielding results with good correlation to ELISA and Gyrolab.
Conclusions:
NULISA technology offers high sensitivity, full automation, and a wide dynamic range, streamlining assay development and optimization, simplifying sample analysis, minimizing errors, and increasing throughput.
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