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Related Experiment Video

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High-resolution awake mouse fMRI at 14 tesla.

David Hike1, Xiaochen Liu1, Zeping Xie1

  • 1Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Harvard Medical School, Massachusetts General Hospital, Charlestown, United States.

Elife
|January 9, 2025
PubMed
Summary

This study presents a novel awake mouse functional magnetic resonance imaging (fMRI) platform using an implantable RF coil and acclimation techniques. It enables high-resolution brain mapping, revealing sensory processing and learned anticipation in awake mice.

Keywords:
BOLDawake mousefMRImouseneurosciencepredictionvibrissa stimulationvisual stimulation

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Medical Imaging

Background:

  • High-resolution awake mouse functional magnetic resonance imaging (fMRI) is challenging due to motion artifacts and stress.
  • Existing methods struggle with image distortion at the air/tissue interface in mouse brains.

Purpose of the Study:

  • To develop an advanced awake mouse fMRI platform for high-resolution brain mapping.
  • To investigate sensory processing and higher-order functions in awake mice.

Main Methods:

  • Designed an implantable radio frequency (RF) surface coil doubling as a headpost to minimize image distortion.
  • Implemented a thorough animal acclimation protocol to reduce motion artifacts.
  • Utilized a 14 T scanner for fMRI at 100 µm resolution with a 2 s sampling rate.

Main Results:

  • Achieved brain-wide functional mapping of visual and vibrissa stimulation in awake mice.
  • Detected blood oxygen level-dependent (BOLD) responses in association cortices, including anterior cingulate cortex and ventral retrosplenial area (VRA).
  • Observed anticipatory BOLD signals in the barrel cortex (BC) preceding vibrissa stimulation, indicating learned anticipation.

Conclusions:

  • Established a high-resolution awake mouse fMRI platform for detailed brain function analysis.
  • Demonstrated the platform's capability to map sensory pathways and higher-order processing in awake mice.
  • Provided evidence for learned anticipation in sensory-associative cortices through advanced fMRI techniques.