Peroxidase 4-Based Enzymatic Synthesis of Stilbene Oligomers in Methyl Jasmonate-Elicited Grapevine Cell Suspensions

Stilbenes are specialized metabolites that are particularly abundant in Vitis species. Although the biosynthetic pathways of stilbenes have been well-characterized, the role of specific peroxidases in stilbene oligomerization remains to be investigated. In this study, we used grapevine cell cultures to characterize the functional role of Vitis vinifera peroxidase 4 (VvPRX4) in the production of resveratrol oligomers after elicitation with methyl jasmonate (MeJA). We showed that MeJA triggers the accumulation of t-resveratrol, resveratrol dimers, and predicted resveratrol trimers in culture medium. This accumulation was correlated with upregulation of the PRX4 gene in grapevine cells. Using bacterial crude extracts containing VvPRX4, we demonstrated that VvPRX4 converts t-resveratrol into dimers, t-ε-viniferin into tetramers, and both combined substrates into resveratrol trimers. Additionally, VvPRX4 mediates the formation of glycosylated dimers using t-piceid and t-resveratrol as substrates. These results highlight the functional role of VvPRX4 in stilbene oligomerization in MeJA-elicited grapevine cell cultures.