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Updated: Jun 3, 2025
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
Published on: November 1, 2024
Generation of a KDM5D knockout human embryonic stem cell line with CRISPR/Cas9 technology
Ouhui Li1, Yuting Zhen1, Chao Sun2
1Key Laboratory of Tropical Translational Medicine of Ministry of Education & Key Laboratory of Brain Science Research Transformation in Tropical Environment of Hainan Province, Department of Biochemistry and Molecular Biology, School of Basic Medicine and Life Sciences, Hainan Medical University, Haikou, Hainan 571199, China; Hainan Provincial Key Laboratory for Human Reproductive Medicine and Genetic Research, Key Laboratory of Reproductive Health Diseases Research and Translation (Hainan Medical University), Ministry of Education, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 571101, China; Department of Reproductive Medicine, Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 571101, China; National Center for International Research "China-Myanmar Joint Research Center for Prevention and Treatment of Regional Major Disease" by the Ministry of Science and Technology of China, Haikou, Hainan 571101, China; Haikou Key Laboratory for Preservation of Human Genetic Resource, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan 571101, China.
Abstract:
KDM5D is a gene implicated in spermatogenic failure and sex-related differences in colon cancer progression, though its role in spermatogenesis remains unclear. We successfully generated a KDM5D knockout human embryonic stem cells using CRISPR/Cas9 technology. This knockout cell line provides a valuable model for studying KDM5D's function in spermatogenesis and its influence on sex differences in various diseases.
Insights
Researchers created a KDM5D knockout stem cell line to study its role in male infertility and sex differences in diseases. This model aids in understanding KDM5D gene function in spermatogenesis.
Area of Science:
- Genetics and Developmental Biology
- Reproductive Medicine
- Oncology
Background:
- The gene KDM5D is linked to spermatogenic failure and sex disparities in colon cancer.
- Its precise function in human spermatogenesis is not well understood.
Purpose of the Study:
- To generate a KDM5D knockout human embryonic stem cell line.
- To establish a model system for investigating KDM5D's role in spermatogenesis.
- To explore KDM5D's contribution to sex differences in disease progression.
Main Methods:
- CRISPR/Cas9 gene editing technology was employed.
- Human embryonic stem cells were utilized to create the knockout model.
Main Results:
- A KDM5D knockout human embryonic stem cell line was successfully generated.
- This cell line serves as a platform for functional studies.
Conclusions:
- The KDM5D knockout stem cell line is a valuable tool for research.
- It facilitates the study of KDM5D's function in male reproductive health.
- It aids in understanding KDM5D's role in sex-specific disease mechanisms.
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