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Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis
Published on: December 8, 2023
Diterpenoid alkaloids from the roots of Aconitum bulbilliferum
Yan-Yan Luo1, Jin-Bu Xu2, Shuai Huang2
1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China; Key laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China.
Abstract:
The phytochemistry study of Aconitum bulbilliferum Hand.-Mazz. is firstly reported. Two C20-diterpenoid alkaloids, named bulbilliferines A and B (1 and 2), three C19-diterpenoid alkaloids, bulbillifedines A-C (3-5), and twenty known compounds (6-25) were isolated from the roots of A. bulbilliferum. The structures of these compounds were determined by the comprehensive spectroscopic analyses (HRESIMS, IR, 1D and 2D NMR). Among them, bulbilliferine A (1) is a denudatine-type diterpenoid alkaloid bearing a 16, 17-epoxy group. At a nontoxic dose of 10 mg/kg, 14-O-anisoylchasmanine (14) hydrochloride and austroconitine B (17) hydrochloride respectively exhibited the excellent analgesic effects with 83.6 % and 83.1 % inhibitions against acetic acid-induced writhing of mice.
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