Related Experiment Video

Updated: Jun 3, 2025

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

224

Research progress in deubiquitinase OTUD3

Dan Hou1, Dan Yu2, Guoshuai Yang3

  • 1Department of Neurology, Haikou Affiliated Hospital of Xiangya School of Medicine, Central South University, Haikou 570208. houtan@163.com.

Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences
|January 9, 2025
PubMed

Insights

OTU domain-containing protein 3 (OTUD3) is a deubiquitinase impacting cell functions and disease. Its roles vary, acting as a tumor suppressor in some cancers and an oncogene in others, highlighting its complex involvement in immunity and disease.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • OTU domain-containing protein 3 (OTUD3) is a deubiquitinase with diverse roles in cellular processes.
  • OTUD3 expression varies significantly across different disease models, indicating its involvement in pathology.

Purpose of the Study:

  • To elucidate the multifaceted roles of OTUD3 in physiological and pathological processes.
  • To understand the molecular mechanisms underlying OTUD3's function in immunity and diseases like cancer and neurodegeneration.

Main Methods:

  • Deubiquitination assays to identify OTUD3 substrates.
  • Analysis of OTUD3 expression patterns in various disease models.
  • Investigating OTUD3's impact on apoptosis, inflammation, cell cycle, and invasion.

Main Results:

  • OTUD3 regulates critical biological functions including apoptosis, inflammation, cell cycle, proliferation, and invasion.
  • OTUD3 exhibits context-dependent roles in cancer, acting as a tumor suppressor in breast, esophageal, colon, and thyroid cancers, but oncogenic in liver and lung cancers.
  • OTUD3 is implicated in innate antiviral immunity, neural development, and neurodegenerative diseases.

Conclusions:

  • OTUD3 is a key regulator in numerous cellular processes and diseases, with significant implications for innate immunity and cancer.
  • OTUD3's dual role in cancer necessitates further research into its specific molecular mechanisms.
  • OTUD3 serves as a potential biomarker for cancer diagnosis and prognosis, and targeted therapies are a future direction.
Keywords:
OTU domain-containing protein 3deubiquitinaseinnate antiviral immunityneurodegenerative diseasestumorubiquitin-proteasome system

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