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Updated: May 6, 2026
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
Published on: November 10, 2016
A simply synthesized, silver ions-doped porous gold microparticles-based SERS aptamer sensor for ultrasensitive and
Bixia Liang1, Zhixi Huang1, Lingyan Cheng1
1Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China.
Background:
The multifunctional cytokine interleukin-6 (IL-6) plays a pivotal role in chronic and acute inflammatory responses, underscoring the importance of accurately determining IL-6 levels for early diagnosis, prevention, and treatment of inflammation.
Results:
This study developed a versatile and innovative single-particle surface-enhanced Raman spectroscopy (SERS) sensing platform for the precise and sensitive quantification of IL-6 in complex samples using a novel one-pot synthesized, silver ions-doped three-dimensional porous gold microparticles (PGMs) with abundant hot spots for robust SERS enhancement. By rationally designing rich cytosine-Ag+-cytosine base pairs between IL-6 aptamers and complementary chains on the PGMs, we harnessed the SERS-enhancing effect to achieve highly sensitive and specific IL-6 quantification within a wide range of 10-9 to 10-4 mg/mL and a limit of detection (LOD) of 0.093 pg/mL. Moreover, the accurate detection of IL-6 in spiked serum samples exhibited a remarkable recovery rate ranging from 97.2 % to 107.6 %.
Significance:
This aptamer sensor provides an alternative tool for cost-effective, sensitive and broad-range IL-6 detection, which would contribute to the early detection of inflammation and pave the way for timely interventions and improved patient outcomes.
