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Updated: Jun 3, 2025

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

Ultrasensitive detection of intact SARS-CoV-2 particles in complex biofluids using microfluidic affinity capture.

Daniel C Rabe^1,2,3,4, Adarsh Choudhury^1,2, Dasol Lee^1,2

¹Krantz Family Center for Cancer Research, Massachusetts General Hospital, Boston, MA, USA.

Science Advances

|January 10, 2025

Summary

A new microfluidic device captures intact SARS-CoV-2 virus from biofluids using ACE2. This technology enables ultrasensitive detection of viral load, aiding in COVID-19 monitoring and management.

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Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Area of Science:

Biomedical Engineering
Virology
Microfluidics

Background:

Detecting viruses like SARS-CoV-2 in complex biofluids is challenging due to interfering biomolecules.
Accurate viral load measurement is crucial for effective disease management and monitoring treatment efficacy.

Purpose of the Study:

To develop an affinity-based microfluidic device for the specific capture and detection of intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
To enable ultrasensitive quantification of viral load in various human biofluids.

Main Methods:

Engineered angiotensin-converting enzyme 2 (ACE2) for specific affinity capture of intact SARS-CoV-2.
Utilized a microfluidic device with a staggered herringbone pattern and nanoparticle surface coating.
Optimized processing conditions for enhanced sensitivity and detection.

Main Results:

Achieved detection of as few as 3 viral copies per milliliter.
Validated the assay on patient samples: 72% of plasma, 36% of saliva, and 29% of stool samples showed SARS-CoV-2 detection.
Demonstrated longitudinal monitoring capability for tracking active viral infections over time.

Conclusions:

The developed microfluidic device offers a highly sensitive method for detecting intact SARS-CoV-2 in complex biofluids.
This technology has potential for widespread application in viral load monitoring and disease management.
The platform is adaptable for detecting other viruses by utilizing relevant cell entry molecules.