Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ADELLE: A global testing method for Trans-eQTL mapping.

bioRxiv : the preprint server for biology·2024
Same author

Overcoming the "feast or famine" effect: improved interaction testing in genome-wide association studies.

bioRxiv : the preprint server for biology·2024
Same author

JASPER: fast, powerful, multitrait association testing in structured samples gives insight on pleiotropy in gene expression.

bioRxiv : the preprint server for biology·2024
Same author

BRASS: Permutation methods for binary traits in genetic association studies with structured samples.

PLoS genetics·2023
Same author

Genome wide association analysis in a mouse advanced intercross line.

Nature communications·2018
Same journal

Genetic survey of biomarkers at early and mid-pregnancy identifies pregnancy-specialized immune regulation.

PLoS genetics·2026
Same journal

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program.

PLoS genetics·2026
Same journal

Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

PLoS genetics·2026
Same journal

Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

PLoS genetics·2026
Same journal

Multiple instance fine-mapping: Predicting causal regulatory variants with a deep sequence model.

PLoS genetics·2026
Same journal

Nuclear ubiquitin-conjugating enzyme TrUbc4 and F-box protein TrFwd1-mediated modification of Cre1 in Trichoderma reesei establishes a regulatory mechanism for carbon catabolite repression.

PLoS genetics·2026
See all related articles

Related Experiment Video

Updated: Jun 26, 2026

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
12:36

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

Published on: October 20, 2010

ADELLE: A global testing method for trans-eQTL mapping.

Takintayo Akinbiyi1, Mary Sara McPeek1,2, Mark Abney2

  • 1Department of Statistics, The University of Chicago, Chicago, Illinois, United States of America.

Plos Genetics
|January 10, 2025
PubMed
Summary
This summary is machine-generated.

A new statistical framework, ADELLE, enhances the discovery of distant genetic variants (trans-eQTLs) that regulate gene expression. This powerful tool uses summary statistics to identify more trans-eQTLs, improving our understanding of genetic regulation.

More Related Videos

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

Related Experiment Videos

Last Updated: Jun 26, 2026

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
12:36

In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme

Published on: October 20, 2010

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

Area of Science:

  • Genetics and Genomics
  • Systems Biology
  • Statistical Genetics

Background:

  • Identifying genetic variants that influence gene expression is crucial for understanding gene regulation.
  • Cis-acting expression quantitative trait loci (cis-eQTLs) are readily detected near genes, but distant trans-acting variants (trans-eQTLs) are challenging to discover despite their significant contribution to heritability.
  • Current statistical methods struggle with the low power and high multiple testing burden required for trans-eQTL mapping.

Purpose of the Study:

  • To develop a powerful statistical testing framework, ADELLE, for identifying trans-eQTLs using only summary statistics.
  • To design ADELLE to be sensitive to single nucleotide polymorphisms (SNPs) associated with multiple gene expression traits, a common characteristic of trans-eQTLs.
  • To demonstrate ADELLE's superior performance compared to existing methods in trans-eQTL discovery.

Main Methods:

  • ADELLE, a novel statistical framework utilizing summary statistics for trans-eQTL mapping.
  • Simulations were conducted to compare ADELLE's power against eight other methods for detecting SNPs associated with 0.1%-2% of 10,000 traits.
  • Application of ADELLE to a mouse advanced intercross line dataset and the eQTLGen dataset for trans-eQTL analysis.

Main Results:

  • In simulations, ADELLE demonstrated superior or comparable power to the highest performing methods across various settings for detecting SNPs associated with multiple traits.
  • ADELLE successfully identified significant trans-eQTLs in mouse data that were missed by standard analyses.
  • In the eQTLGen dataset, ADELLE revealed additional trans associations for 1,451 previously identified trans-eQTLs, highlighting its ability to uncover novel regulatory relationships.

Conclusions:

  • ADELLE is a powerful and effective statistical tool for discovering trans-eQTLs, particularly those with small effect sizes and associations with multiple traits.
  • The framework's ability to leverage summary statistics makes it broadly applicable for large-scale genetic studies.
  • ADELLE significantly advances the identification of trans regulators, offering new insights into the complex genetic architecture of gene expression.