Exogenous melatonin enhances heat tolerance in buckwheat seedlings by modulating physiological response mechanisms

Melatonin (MT) serves as a potent antioxidant in plant organisms, bolstering their resilience to temperature stress. In this study, the impact of MT on various buckwheat varieties under high-temperature stress conditions (40 °C) was investigated. Specifically, five buckwheat seedling varieties, comprising three sweet buckwheat variants (Fagopyrum esculentum) and two bitter buckwheat types (Fagopyrum tataricum), were subjected to foliar sprays of melatonin at concentrations of 50, 100 and 200 μM, with water at 25 °C employed as a control. Results demonstrated that exogenous MT at different concentrations improved the growth and physiological parameters of buckwheats, ameliorating damage induced by high-temperature stress. Notably, the application of 100 μM MT significantly augmented shoot biomasses of buckwheat seedlings under high-temperature conditions. Furthermore, the MT significantly increased the levels of osmotic adjustment substances and chlorophyll concentrations, enhanced antioxidant enzyme activities, chlorophyll fluorescence parameters, and improved photosynthetic gas exchange parameters in five different varieties of buckwheat. This led to the alleviation of damage to buckwheat seedlings subjected to high-temperature stress. Subsequently, five advanced statistical analysis methods: Principal Component Analysis, Grey Relational Analysis, Path Coefficient Analysis, Membership Function Method, and Coupling Coordination Analysis were employed to delve deeper into the existing data indicators. To summarize, the beneficial effect of exogenous melatonin on seedling growth is primarily achieved through the coordination and regulation of the antioxidant enzyme system and osmotic regulatory substances, ensuring the growth and development of buckwheat seedlings while also improving their heat tolerance. The treatment with a concentration of 100 μM of MT was the most effective.