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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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Related Experiment Video

Updated: Jun 3, 2025

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
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Automated high-throughput RP-HPLC-MS and SFC-MS analytical and purification platforms to support drug discovery.

Kuanchang Chen1, José Luís Dores-Sousa2, Alberto Fontana3

  • 1Chemistry Capabilities, Analytical & Purification, Global Discovery Chemistry. Janssen Research & Development, a Division of Janssen Pharmaceuticals, Johnson & Johnson company, 1400 McKean Rd. Spring House PA 19477, USA.

Journal of Chromatography. A
|January 10, 2025
PubMed
Summary

Automated High-Throughput Purification (HTP) workflows, integrating SAPIO LIMS and Analytical Studio, accelerate drug discovery. This system streamlines sample analysis and handling, significantly reducing Design-Make-Test-Analyze cycles.

Keywords:
AutomationChromatographyHPLC-MS, SFC-MSLaboratory information management system (LIMS)MicroscalePeptidesReversed-phase, Small molecules

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Area of Science:

  • Pharmaceutical Sciences
  • Analytical Chemistry
  • Laboratory Automation

Background:

  • The pharmaceutical industry faces pressure to accelerate drug discovery.
  • Automated workflows, known as High-Throughput Purification (HTP), are gaining traction.
  • Integrating Laboratory Information Management Systems (LIMS) with HTP is crucial for efficiency.

Purpose of the Study:

  • To describe the customization and implementation of SAPIO LIMS for automated HTP workflows at Janssen R&D.
  • To detail a comprehensive HTP workflow from crude analysis to compound submission for biological assays.
  • To highlight the integration of Analytical Studio™ for data processing within the HTP workflow.

Main Methods:

  • Customization of SAPIO LIMS for automated HTP workflows.
  • Integration of Analytical Studio™ for data processing.
  • Utilization of robotic platforms for sample handling and automation for chromatographic (RP-LC-MS, SFC-MS) and Nuclear Magnetic Resonance (NMR) analyses.

Main Results:

  • Successful implementation of an integrated HTP workflow combining SAPIO LIMS and Analytical Studio™.
  • Streamlined sample handling from crude analysis to redissolution and delivery to Compound Logistics.
  • Increased productivity for HTP teams, leading to reduced Design-Make-Test-Analyze (DMTA) cycles since 2020.

Conclusions:

  • The integrated HTP workflow using SAPIO LIMS and Analytical Studio™ significantly enhances drug discovery efficiency.
  • This represents a novel, complete HTP workflow incorporating LIMS, various analytical techniques, and automated sample handling.
  • The system is ready for biological assay distribution, impacting the overall drug development timeline.