In situ nick translation of human and mouse chromosomes detected with a biotinylated nucleotide

In situ nick translation of human and mouse chromosome preparations has provided further evidence that DNase I sensitivity correlates with transcriptionally active chromatin. Chromosomes from differentiating cells and particular chromosome regions, known to differ in transcriptional activity, generally reveal predicted differences in relative DNase I sensitivity. The application of a biotinylated nucleotide in these studies provides a new level of cytological resolution and offers the potential for further refinement.