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Updated: Oct 13, 2026

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

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In situ nick translation of human and mouse chromosomes detected with a biotinylated nucleotide

M L Murer-Orlando, A C Peterson

    Experimental Cell Research
    |April 1, 1985
    PubMed

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    In-situ Hybridization02:31

    In-situ Hybridization

    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
    Types of probes and labels
    A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...