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Metabolic studies of Ogataea polymorpha using nine different corn steep liquors
Sekar Mayang W Wahjudi1, Dominik Engel1, Jochen Büchs2
1Aachener Verfahrenstechnik - Biochemical Engineering, RWTH Aachen University, Forckenbeckstr. 51, 52074, Aachen, Germany.
BMC Biotechnology
|January 10, 2025
Summary
Corn steep liquor (CSL) offers a cost-effective nutrient source for Ogataea polymorpha fermentation. Batch-to-batch variations in CSL can be detected using Respiration Activity Monitoring System (RAMOS) and microtiter plates (µTOM) for optimized cultivation.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Bioprocess Engineering
Background:
- Rising demand for cost-effective nutrient sources in fermentation.
- Corn steep liquor (CSL) as a promising alternative nutrient source derived from waste streams.
- Challenges associated with CSL: batch-to-batch variations and compositional inconsistencies.
Purpose of the Study:
- To develop a
- fingerprinting
- system for detecting batch-to-batch variations in CSL.
- To optimize Ogataea polymorpha cultivations using CSL.
Main Methods:
- Utilized the Respiration Activity Monitoring System designed for shake flasks (RAMOS).
- Employed 96-well microtiter plates (µTOM) for high-throughput screening.
- Performed targeted nutrient supplementation to identify limitations.
Main Results:
- Identified ammonium nitrogen as a limiting substrate in CSL, which was overcome by ammonium sulphate supplementation.
- Observed morphological changes and altered cell size distribution in O. polymorpha under ammonium limitation.
- Determined that increased CSL storage time negatively impacts cultivation due to micronutrient precipitation (copper, iron, zinc).
Conclusions:
- CSL is a versatile nutrient source for O. polymorpha.
- "Fingerprinting" CSL batches enables early screening and selection of optimal batches.
- Fermentation inconsistencies can be mitigated by selecting superior CSL batches or by targeted supplementation of inferior batches.
Keywords:
Ogataea polymorphaBatch-to-batch variationCorn steep liquorFingerprintingMedia supplementationMorphology changeOnline monitoringRAMOSµTOMMore Related Videos
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