Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

10.5K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
10.5K
Inflammatory Bowel Disease I: Ulcerative Colitis01:27

Inflammatory Bowel Disease I: Ulcerative Colitis

135
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
135
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

11.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing maternal age worsens pregnancy outcomes following single frozen embryo transfer with preimplantation genetic testing.

Reproductive biomedicine online·2026
Same author

Trigger-day hCG effects on DNA methylation and neurodevelopment in ART offspring.

Nature communications·2026
Same author

Interventions for Silent Brain Infarction and Perioperative Neurocognitive Disorders in Cardiovascular Surgery (INSPIRE): study protocol for a multicentre randomised controlled trial.

BMJ open·2026
Same author

Comment on: 'Treatment patterns and long-term outcomes in anti-VEGF-treated macular oedema secondary to retinal vein occlusion: a retrospective observational study'.

Eye (London, England)·2026
Same author

Spatially interpretable artificial intelligence framework to tailored neoadjuvant dual HER2 blockade in HER2-positive breast cancer.

Signal transduction and targeted therapy·2026
Same author

Socioeconomic disparities and health-related parenting practices in shaping early infant neurodevelopment: evidence from a Chinese prospective birth cohort.

BMC pregnancy and childbirth·2026

Related Experiment Video

Updated: Jun 3, 2025

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

935

Disrupted mitochondrial morphology and function exacerbate inflammation in elderly-onset ulcerative colitis.

Mengmeng Zhang1, Hong Lv1, Xiaoyin Bai1

  • 1Department of Gastroenterology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Immunity & Ageing : I & A
|January 10, 2025
PubMed
Summary

Mitochondrial damage is more severe in elderly ulcerative colitis (UC) patients, contributing to worse inflammation. This study highlights mitochondrial dysfunction

Keywords:
ElderlyImmune infiltrationInflammatory responseMitochondrial damageUlcerative colitis

More Related Videos

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

12.2K
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
05:41

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Published on: April 6, 2022

2.8K

Related Experiment Videos

Last Updated: Jun 3, 2025

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

935
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

12.2K
Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model
05:41

Therapeutic Evaluation of Fecal Microbiota Transplantation in an Interleukin 10-Deficient Mouse Model

Published on: April 6, 2022

2.8K

Area of Science:

  • Gastroenterology
  • Immunology
  • Aging Research

Background:

  • Ulcerative colitis (UC) presents differently in elderly individuals compared to younger populations.
  • Mitochondrial injury is recognized as a critical factor in both aging and inflammatory processes.
  • This research investigates the specific role of mitochondrial damage in the development of UC in adult and elderly-onset patients.

Purpose of the Study:

  • To elucidate the role of mitochondrial damage in the pathogenesis of adult- and elderly-onset ulcerative colitis (UC).
  • To identify differences in mitochondrial function and gene expression between adult and elderly UC patients.
  • To explore the link between mitochondrial injury, immune response, and clinical outcomes in elderly UC.

Main Methods:

  • RNA-sequencing of colonic mucosa from adult and elderly UC patients to identify mitochondria-related differentially expressed genes (mDEGs).
  • Induction of colitis in young and aged mice using dextran sodium sulfate (DSS) to assess mitochondrial morphology and ATP levels.
  • In vitro studies using HCT116 cells to investigate the association of key mDEGs with reactive oxygen species (ROS), oxygen consumption, and the NLRP3/IL-1β pathway.

Main Results:

  • Thirty mDEGs were identified, primarily linked to mitochondrial respiratory function and immune cell infiltration.
  • Aged mice with DSS-induced colitis showed more severe inflammation, mitochondrial damage, and lower ATP levels compared to young mice.
  • ALDH1L1 was identified as a key downregulated gene in UC patients and colitis models, with knockdown affecting ROS, NLRP3, and IL-1β expression in cell lines.

Conclusions:

  • Significant differences in mitochondrial structure, ATP production, and gene expression exist between adult and elderly-onset UC.
  • Mitochondrial damage is a potential key factor contributing to the more severe inflammatory response and poorer outcomes observed in elderly-onset UC.
  • These findings suggest a link between mitochondrial dysfunction, cytokine pathways, and the immune microenvironment in elderly UC patients.