Characterization of Complete Mitochondrial Genome and Phylogeny of Three Echeneidae Species

Species of the family Echeneidae are renowned for their capacity to adhere to various hosts using a sucking disc. This study aimed to examine the mitochondrial genome characteristics of three fish species (Echeneis naucrates, Remora albescens, and Remora remora) within the family Echeneidae and determine their phylogenetic relationships. The findings revealed that the mitochondrial genome lengths of the three species were 16,611 bp, 16,648 bp, and 16,623 bp, respectively, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a D-loop region. Most PCGs utilized ATG as the initiation codon, while only cox I used the GTG as the initiation codon. Additionally, seven genes employed incomplete termination codons (T and TA). The majority of PCGs in the three species displayed negative AT-skew and GC-skew values, with the GC-skew amplitude being greater than the AT-skew. The Ka/Ks ratios of the 13 PCGs did not exceed 1, demonstrating these species had been subjected to purification selection. Furthermore, only tRNA-Ser (GCT) lacked the D arm, while other tRNAs exhibited a typical cloverleaf secondary structure. Bayesian inference (BI) and maximum likelihood (ML) methods were utilized to construct a phylogenetic tree of the three species based on the 13 PCGs. Remora remora was identified as a distinct group, while R. osteochir and R. brachyptera were classified as sister taxa. This study contributes to the mitochondrial genome database of the family Echeneidae and provides a solid foundation for further systematic classification research in this fish group.