Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioremediation00:46

Bioremediation

18.2K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
18.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protective agents to preserve lactic acid bacteria cell viability in type III sourdough dried by spray- and freeze-drying, and the influence on bread production.

Journal of food science and technology·2026
Same author

Pea Protein and Maltodextrin-Based Encapsulation of Anthocyanins From Black Rice Bran: Characterization, Storage Stability, and In Vitro Anthocyanin Release.

Journal of food science·2026
Same author

Key factors affecting cold plasma composition: influence on plasma-activated water and food safety applications.

Critical reviews in food science and nutrition·2026
Same author

Editorial Overview of Special Issue "XXIX CBCTA: Innovation, health, and sustainability in the food production chain".

Food research international (Ottawa, Ont.)·2026
Same author

Study of the Probiotic Potential of Lactic Acid Bacteria Isolated from Artisanal Colonial Cheese and Evaluation of Microencapsulation as a Protective Method Under Simulated Gastrointestinal Conditions.

Foods (Basel, Switzerland)·2026
Same author

<i>Bombyx mori</i> Pupae as a Novel Ingredient from an Underutilized Sericulture Product: A Dual Approach Based on Sustainable Extractions and Sample Pretreatment Strategies.

ACS sustainable chemistry & engineering·2026

Related Experiment Video

Updated: Jun 3, 2025

Extraction and Analysis of Taiwanese Green Propolis
06:50

Extraction and Analysis of Taiwanese Green Propolis

Published on: January 7, 2019

8.4K

Biorefining Brazilian Green Propolis: An Eco-Friendly Approach Based on a Sequential High-Pressure Extraction for

Guilherme Dallarmi Sorita1,2, Wilson Daniel Caicedo Chacon1,2, Monique Martins Strieder1,3

  • 1Foodomics Laboratory, Institute of Food Science Research (CIAL) (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, Spain.

Molecules (Basel, Switzerland)
|January 11, 2025
PubMed
Summary

This study optimized supercritical fluid extraction (SFE) for Brazilian green propolis, achieving high yields and antioxidant activity. A sequential high-pressure extraction (HPE) method proved more sustainable and efficient than low-pressure extraction (LPE).

Keywords:
bio-based solventenvironmental impactnatural productsphytochemicalsprocess optimization

More Related Videos

Extraction of Lignin with High &#946;-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield
10:18

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

20.4K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.2K

Related Experiment Videos

Last Updated: Jun 3, 2025

Extraction and Analysis of Taiwanese Green Propolis
06:50

Extraction and Analysis of Taiwanese Green Propolis

Published on: January 7, 2019

8.4K
Extraction of Lignin with High &#946;-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield
10:18

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

20.4K
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
08:56

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Published on: August 16, 2018

17.2K

Area of Science:

  • Natural Products Chemistry
  • Green Extraction Technologies
  • Phytochemistry

Background:

  • Propolis is a resinous mixture produced by honeybees, valued for its diverse bioactive compounds.
  • Efficient and sustainable extraction methods are crucial for maximizing the recovery of propolis' beneficial constituents.
  • Brazilian green propolis is rich in phenolic compounds with significant antioxidant and anti-inflammatory properties.

Purpose of the Study:

  • To optimize supercritical fluid extraction (SFE) parameters for Brazilian green propolis.
  • To develop and evaluate a sequential high-pressure extraction (HPE) process.
  • To compare the sustainability and efficiency of HPE versus low-pressure extraction (LPE) methods.

Main Methods:

  • Supercritical fluid extraction (SFE) with optimized parameters (60 °C, 21.11% CPME co-solvent).
  • Sequential extraction combining SFE, pressurized liquid extraction (PLE), and subcritical water extraction (SWE) for HPE.
  • Comparison of HPE with a sequential low-pressure extraction (LPE) using a Soxhlet extractor.
  • Gas chromatography-mass spectrometry (GC-MS) for metabolite profiling.
  • Assays for yield, total phenolic content (TPC), antioxidant capacity, and enzyme inhibition (LOX, AChE, BChE).

Main Results:

  • Optimized SFE parameters maximized yield, TPC, antioxidant capacity, and LOX inhibition.
  • GC-MS identified 40 metabolites in SFE extracts, including fatty acids, terpenoids, phenolics, and sterols.
  • HPE achieved a significantly higher overall yield (80.86%) compared to LPE (71.43%).
  • LPE extracts showed superior acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and LOX inhibition.
  • HPE demonstrated greater sustainability and alignment with green chemistry principles.

Conclusions:

  • Optimized SFE is effective for extracting bioactive compounds from Brazilian green propolis.
  • The sequential HPE process offers a more sustainable and higher-yielding alternative to LPE.
  • The neuroprotective and anti-inflammatory activities may result from a synergistic effect of various compounds extracted by different methods.