Understanding the Impact of Salt Stress on Plant Pathogens Through Phenotypic and Transcriptomic Analysis

For plant diseases to become established, plant pathogens require not only virulence factors and susceptible hosts, but also optimal environmental conditions. The accumulation of high soil salinity can have serious impacts on agro-biological ecosystems. However, the interactions between plant pathogens and salinity have not been fully characterized. This study investigated the effects of salt stress on representative plant pathogens, such as Burkholderia gladioli, Burkholderia glumae, Pectobacterium carotovorum subsp. carotovorum (Pcc), Ralstonia solanacearum, and Xanthomonas oryzae pv. oryzae. Phenotypic assays revealed that B. gladioli and R. solanacearum are highly sensitive to salt stress, exhibiting significant reductions in growth, motility, and enzyme production, whereas Pcc showed notable tolerance. Pan-genome-based comparative transcriptomics identified co-downregulated patterns in B. gladioli and R. solanacearum under stress conditions, indicating the suppression of bacterial chemotaxis and type III secretion systems. Uniquely upregulated patterns in Pcc were associated with enhanced survival under high salinity, such as protein quality control, osmotic equilibrium, and iron acquisition. Additionally, the application of salt stress combined with the beneficial bacterium Chryseobacterium salivictor significantly reduced tomato wilt caused by R. solanacearum, suggesting a potential management strategy. This study underscores practical implications for effectively understanding and controlling plant pathogens under future climate changes involving salt stress.