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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Atomic Orbitals02:44

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An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
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Using the Radial Distribution Function to Analyze Atomic Force Microscopy Images of Colloidal Systems.

Sergey V Kraevsky1, Anastasia A Valueva1, Maria O Ershova1

  • 1Institute of Biomedical Chemistry, Pogodinskaya Str., 10, Moscow 119121, Russia.

International Journal of Molecular Sciences
|January 11, 2025
PubMed
Summary

Researchers explored how immobilizing gold nanoparticles (AuNPs) and proteins on a surface reveals their solution behavior. Atomic force microscopy and radial distribution function (RDF) analysis showed AuNPs form short-range ordered structures, while proteins distribute randomly.

Keywords:
Poisson distributionatomic force microscopy (AFM)colloidal particlesgold nanoparticlehorseradish peroxidase (HRP)radial distribution function (RDF)

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Area of Science:

  • Colloid and Surface Science
  • Nanotechnology
  • Biophysics

Background:

  • Biomacromolecules function in aqueous environments, but their behavior in solution is challenging to study directly.
  • Investigating molecules adsorbed on 2D surfaces offers insights into their 3D solution states.
  • Atomic force microscopy (AFM) requires immobilizing nanosized objects for study.

Purpose of the Study:

  • To determine if surface-adsorbed biomolecule structure properties reflect their 3D colloidal solution state.
  • To analyze the structural organization of immobilized gold nanoparticles (AuNPs) and horseradish peroxidase (HRP) on mica.
  • To apply radial distribution function (RDF) methods to assess order in immobilized particle arrangements.

Main Methods:

  • Atomic Force Microscopy (AFM) for imaging immobilized nanoparticles and molecules.
  • Radial Distribution Function (RDF) analysis to quantify particle arrangement and order.
  • Immobilization of gold nanoparticles (AuNPs) and horseradish peroxidase (HRP) on mica surfaces.

Main Results:

  • Gold nanoparticles (AuNPs) exhibit mobility on mica in the presence of water.
  • Immobilized AuNPs form amorphous structures with evident short-range order.
  • Horseradish peroxidase molecules adsorb randomly, with surface density following a Poisson distribution.

Conclusions:

  • Surface immobilization and RDF analysis can provide insights into the structural properties of colloidal systems.
  • The behavior of AuNPs on a surface differs from their behavior in bulk solution.
  • Protein adsorption patterns can be statistically described, indicating random surface distribution.