An Overview of the Current State of Cell Viability Assessment Methods Using OECD Classification.

Area of Science:

• Cell biology
• Toxicology
• Biotechnology

Background:

• Numerous cell viability assessment methods exist, posing challenges for researchers in method selection.
• The Organisation for Economic Co-operation and Development (OECD) classification is crucial for regulatory purposes.
• Existing classifications may not encompass all novel cell viability techniques.

Purpose of the Study:

• To review and categorize existing cell viability assessment methods.
• To assist researchers in choosing appropriate methods based on experimental needs.
• To evaluate the OECD classification and suggest potential improvements.

Main Methods:

• Literature review of cell viability assessment techniques.
• Categorization of methods based on the OECD classification framework.
• Inclusion of novel methods, such as those assessing cell membrane potential.

Main Results:

• Cell viability methods can be broadly classified into four OECD categories: non-invasive cell structure damage, invasive cell structure damage, cell growth, and cellular metabolism.
• Two novel methods based on cell membrane potential fall outside the current OECD classification.
• Each method possesses distinct advantages and disadvantages impacting experimental outcomes.

Conclusions:

• A comprehensive understanding of cell viability methods and their classifications is essential for accurate experimental design.
• The OECD classification provides a valuable regulatory framework but requires updates to include emerging techniques.
• Researchers are guided to select optimal cell viability assays by considering method principles, advantages, and limitations.