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Related Experiment Video

Updated: Jul 19, 2026

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
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A Unique Expression Profile Responding to Powdery Mildew in Wild Emmer Wheat D430.

Yintao Dai1, Ningning Yu1, Hongxing Xu2

  • 1Yantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai 264005, China.

International Journal of Molecular Sciences
|January 11, 2025
PubMed
Summary

Wild emmer wheat accession D430 provides a new source of powdery mildew resistance. This resistance is controlled by the Pm4b gene, offering potential for developing disease-resistant wheat cultivars.

Keywords:
BSR-Seq analysisgene expressionmappingpowdery mildewwild emmer wheat

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Area of Science:

  • Plant Pathology
  • Genetics
  • Wheat Breeding

Background:

  • Powdery mildew, caused by *Blumeria graminis* f. sp. *tritici* (*Bgt*), significantly impacts global wheat production.
  • Wild emmer wheat (*Triticum dicoccoides*) possesses valuable genetic resources for disease resistance.
  • Developing wheat cultivars with enhanced resistance is crucial for disease management.

Purpose of the Study:

  • To identify and characterize the genetic basis of powdery mildew resistance in wild emmer wheat accession D430.
  • To map the resistance gene and understand its molecular identity.
  • To explore potential molecular targets for enhancing wheat resistance.

Main Methods:

  • Genetic analysis of crosses between resistant (D430) and susceptible wheat lines.
  • Bulked Segregant Analysis by Sequencing (BSR-Seq) for gene mapping.
  • Sequence alignment and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
  • Quantitative Real-Time PCR (qRT-PCR) for gene expression analysis.

Main Results:

  • Powdery mildew resistance in D430 was controlled by a single dominant locus, tentatively named *PmD430*.
  • Molecular mapping and sequence analysis confirmed *PmD430* is identical to the *Pm4b* gene.
  • Differential gene expression analysis identified 1871 DEGs, with eight disease-related genes showing unique expression patterns upon *Bgt* infection.

Conclusions:

  • The wild emmer wheat accession D430 harbors the *Pm4b* gene, conferring strong resistance to powdery mildew.
  • The identified differentially expressed genes present potential targets for future strategies to improve wheat powdery mildew resistance.