An Innovative Method of Improving an Extract of Andrographis paniculata from Leaves: Its Anticancer Effect Involves...

In this manuscript, the effects of two extracts from Andrographis paniculata were tested: (a) an extract titrated to 49.7% of andrographolide and obtained from leaves of the plant: (b) the pure andrographolide titrated to 99%. The extracts were dissolved in 1-butanol and tested on tumor lines (MCF7 and SH-SY5Y) and the non-tumor line (Huvec) to understand the effects on cell proliferation. The addition of a sonication process improved their dissolution and efficacy making these extracts unique and innovative. The experiments conducted (viability measurements, solubility of the extracts, IC₅₀ tests, measurement of oxidative potential, lipid and cytosolic calcium concentration, and mortality assessment by annexin assay) showed a different behavior of the extracts on cancer cells and not. In particular, the extracts did not cause toxic effects on the viability of the Huvec cells, while both tumor lines were damaged, demonstrating that cancer cells are more susceptible to extracts of A. paniculata than healthy cells. The mechanism of action responsible for the damage detected involved the functioning of the endoplasmic reticulum organelle and finally resulted in apoptotic death. For this reason, the extracts considered have shown a potential anti-tumor role and A. paniculata could be used and exploited in pharmacological therapy against cancer. However, further studies, obtained in clinical practice, should be conducted to increase knowledge of the effects of A. paniculata on the organism and its phytotherapeutic role.