Circadian- and Light-Driven Rhythmicity of Interconnected Gene Networks in Olive Tree

A circadian clock (CC) has evolved in plants that synchronizes their growth and development with daily and seasonal cycles. A properly functioning circadian clock contributes to increasing plant growth, reproduction, and competitiveness. In plants, continuous light treatment has been a successful approach for obtaining novel knowledge about the circadian clock. The olive tree (Olea europaea L.) is one of the most important crops in the Mediterranean area, and, so far, limited information is available on its CC gene network. Here, we studied the behavior of circadian rhythm genes under LD (light/darkness) and LL (light/light) conditions, the relationships in this network, and the ability of the treatments to modulate gene expression in the photoprotective pigment and lipid biosynthesis pathways. One month of LL conditions increased olive growth performance, but LL exposure also caused reductions in vegetative growth and chlorophyll accumulation. A panel was designed for a study of the transcription expression levels of the genes involved in light perception, the CC, and secondary metabolite and fatty acid biosynthesis. Our results revealed that the levels of 78% of the transcripts exhibited intraday differences under LD conditions, and most of them retained this rhythmicity after exposure to one and two months of LL conditions. Furthermore, co-regulation within a complex network among genes of photoreceptors, anthocyanidins, and fatty acids biosynthesis was orchestrated by the transcription factor HY5. This research enriches our knowledge on olive trees grown under prolonged irradiation, which may be attractive for the scientific community involved in breeding programs for the improvement of this species.