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Transcriptomic and Proteomic Analysis of Monkeypox Virus A5L-Expressing HEK293T Cells
Mingzhi Li1,2,3, Jiaqi Xiong1,2,3, Hao Zhou3
1Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330000, China.
International Journal of Molecular Sciences
|January 11, 2025
Summary
Monkeypox virus (MPXV) A5L protein significantly impacts host cell functions, including the cell cycle and translation. This study reveals key interactions between MPXV A5L and host ribosomal proteins, offering insights into viral replication mechanisms.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Monkeypox virus (MPXV) is a significant zoonotic threat within the Orthopoxvirus genus.
- Limited understanding exists regarding MPXV's clinical manifestations and molecular interactions within host cells.
Purpose of the Study:
- To investigate the host cell response to the MPXV A5L protein using transcriptomic and proteomic approaches.
- To elucidate the role of MPXV A5L in viral-host interactions and cellular processes.
Main Methods:
- High-throughput RNA sequencing (RNA-seq) to analyze gene expression changes in HEK293T cells expressing MPXV A5L.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify host proteins interacting with MPXV A5L.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
Main Results:
- MPXV A5L expression altered 1473 genes (911 upregulated, 562 downregulated) in HEK293T cells.
- 185 host proteins interacting with MPXV A5L were identified, predominantly ribosomal and cytoskeletal proteins.
- Transcriptomic analysis indicated modulation of cell cycle, ribosome, and DNA replication pathways.
Conclusions:
- MPXV A5L plays a crucial role in viral internalization and assembly.
- The MPXV A5L protein significantly affects the host's translation system.
- Combined transcriptomic and proteomic data provide novel insights into MPXV-host interplay.
