A rapid RIA for LH in serum and first morning void urine--application in an AID programme

A rapid luteinizing hormone (LH) radioimmunoassay, with a 40-min incubation time, has been described and was used to measure the serum and urinary LH profiles in 92 menstrual cycles. A comparison of LH concentrations in first morning voiding (FMV) urine samples demonstrated that the pre-ovulatory LH peak in urine could be detected within 24 h of that in serum and that its offset was somewhat prolonged. FMV urine samples may be used as a convenient alternative to serum for the monitoring of artificial insemination by donor (AID) cycles.