Related Experiment Video

Updated: Jun 3, 2025

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
09:40

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Published on: June 20, 2018

17.4K

Proteinuria and tubular cells: Plasticity and toxicity

Anna Faivre1,2, Thomas Verissimo1, Sophie de Seigneux1,2

  • 1Department of Medicine, Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland.

Acta Physiologica (Oxford, England)
|January 11, 2025
PubMed
Abstract

Insights

Proteinuria, a key factor in chronic kidney disease (CKD) progression, damages kidney tubular epithelial cells. Understanding these effects reveals new therapeutic targets for CKD.

Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • Proteinuria is a primary indicator of chronic kidney disease (CKD) progression.
  • While glomerular damage initiates proteinuria, tubular epithelial cells are significantly impacted, driving disease advancement.
  • Interventions reducing proteinuria are currently the most effective nephroprotective strategies.

Purpose of the Study:

  • To elucidate mechanisms of tubular protein handling.
  • To explore the detrimental effects of proteinuria on tubular epithelial cell function.
  • To review the dynamic phenotypic changes in nephrons due to proteinuria.

Main Methods:

  • This study is a narrative review.
  • Literature search conducted on PubMed from inception to 2024.
  • Focus on the impact of proteinuria on tubular cells.

Main Results:

  • Proteinuria induces toxic effects on tubular epithelial cells via signaling pathways, ER stress, oxidative stress, and metabolic changes.
  • Plasma proteins trigger detrimental cellular responses.
  • The review details phenotypic alterations in nephrons caused by proteinuria.

Conclusions:

  • The interaction between proteinuria and tubular cells is critical for CKD progression.
  • Identifying these mechanisms offers potential therapeutic targets for kidney disease.
  • This review provides insights into novel therapeutic strategies for managing CKD.
Keywords:
CKDfibrosisinflammationproteinuriaproximal tubular cellsspatial resolution

More Related Videos

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

10.9K
Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection10:25

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

1.7K

Related Concept Videos

Physiology of Urine Formation01:24

Physiology of Urine Formation

Urine formation is an essential function of the human body. It plays a critical role in maintaining homeostasis by regulating the volume and composition of body fluids. The kidneys, the primary organs involved in this process, filter blood to remove waste products and excess substances, ultimately producing urine.
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
2.9K
Renal Tubule and Collecting Duct01:24

Renal Tubule and Collecting Duct

The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
760
Tubular Reabsorption and Secretion01:28

Tubular Reabsorption and Secretion

Tubular secretion and reabsorption are two critical processes in the nephron tubule of the kidneys. When the fluid filtered from the glomerulus enters the proximal convoluted tubule, it is referred to as filtrate, and its composition changes due to tubular reabsorption and secretion.
Tubular reabsorption is a selective process that starts when the filtrate enters the proximal tubules. It involves substances traveling through the transcellular route (through the tubule cell and peritubular...
1.5K
Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
2.1K
Drug Elimination by Renal Route: Tubular Secretion01:15

Drug Elimination by Renal Route: Tubular Secretion

Once the process of glomerular filtration is completed, blood carrying unfiltered drug molecules traverses through efferent arterioles and makes its way into the peritubular capillaries in the proximal tubule. A variety of carriers play a pivotal role in actively secreting drugs from these peritubular capillaries into the tubular fluid. The organic anion transporter transfers acidic drugs, against an electrochemical gradient, from the peritubular capillaries into the renal tubule cells and...
2.1K
Renal Drug Excretion: Tubular Secretion01:28

Renal Drug Excretion: Tubular Secretion

Active tubular secretion is a robust, energy-demanding process that utilizes carrier systems to transport drugs into renal tubules. The active renal secretion systems include the organic anion transporter (OAT) for weak acids and the organic cation transporter (OCT) for weak bases. Structurally similar drugs can compete for the same transporter, potentially leading to drug accumulation and toxicity. However, this principle can be exploited therapeutically. One example is probenecid (Probalan),...
117