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Updated: May 10, 2026

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
Multicenter In-House Evaluation of an Amplicon-Based Next-Generation Sequencing Panel for Comprehensive Molecular
Eloisa Jantus-Lewintre1, Alessandra Rappa2, Dina Ruano3
1Fundación Investigación Hospital General Universitario de València, Universitat Politècnica de València, CIBERONC, Valencia, Spain.
The Oncomine™ Comprehensive Assay Plus (OCA Plus) panel accurately profiles solid tumors for genetic biomarkers. This multicenter study confirms its high reproducibility and concordance with existing methods for targeted cancer therapies.
Area of Science:
- Oncology
- Genomics
- Biomarker Discovery
Background:
- Predicting response to targeted cancer therapies relies on genetic biomarkers.
- Comprehensive genomic profiling (CGP) requires evaluation for accuracy and reproducibility.
Purpose of the Study:
- To conduct a multicenter evaluation of the Oncomine™ Comprehensive Assay Plus (OCA Plus) Pan-Cancer Research Panel.
- To assess the correlation, concordance, and reproducibility of the OCA Plus panel for CGP of solid tumors.
Main Methods:
- Analysis of 193 research samples (125 DNA, 68 RNA) across five European research centers.
- Evaluation of the OCA Plus panel against orthogonal methods for various genetic biomarkers.
- Assessment of reproducibility using shared, pre-characterized tissue biopsies.
Main Results:
- High success rates for DNA (96.6%) and RNA (89.7%) sequencing.
- Detailed genomic profiling including single nucleotide variants/indels, copy number variants, fusions, microsatellite instability, tumor mutational burden, and homologous recombination deficiency.
- High concordance rates for most biomarkers (e.g., 94.8% for SNVs/indels, 96.5% for CNVs, 100% for HRD) and excellent reproducibility across centers.
Conclusions:
- The OCA Plus panel demonstrates high reproducibility and accuracy in a multicenter setting.
- Confirms findings from previous single-center studies, supporting its utility for comprehensive genomic profiling.
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