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Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

841
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
841

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Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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Protocol for assessing immune-target cell interactions using a single-cell cytotoxicity assay.

Zhihao Wei1, Konglan Lin1, Min Huang1

  • 1Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China; Breast Tumor Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.

STAR Protocols
|January 11, 2025
PubMed
Summary

This study introduces a new method using microwell arrays and automated microscopy for real-time observation of natural killer (NK) cell cytotoxicity. This technique allows for detailed, single-cell level analysis of immune cell interactions and their dynamic cytotoxic processes.

Keywords:
CancerCell-based AssaysClinical ProtocolImmunology

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Droplet-based Cytotoxicity Assay to Assess Chimeric Antigen Receptor T cells at the Single-cell Level
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Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Standard flow cytometry assays for cytotoxicity lack dynamic process monitoring.
  • Observing immune effector cell-target interactions in real-time is challenging.

Purpose of the Study:

  • To present a protocol for continuous observation of natural killer (NK) cell-mediated cytotoxicity.
  • To enable single-cell level analysis of dynamic immune-target cell interactions.

Main Methods:

  • Utilizing microwell arrays with automated microscopy for continuous monitoring.
  • Isolation and labeling of primary NK cells.
  • Loading cells onto microwell arrays and image analysis.

Main Results:

  • The protocol facilitates the continuous observation of NK cell-mediated cytotoxicity.
  • Enables detailed analysis of immune-target cell interactions at the single-cell level.

Conclusions:

  • This protocol offers a novel approach for studying the dynamics of cytotoxicity.
  • Provides a valuable tool for understanding NK cell function and immune responses.