Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Design considerations for a fluorophotometer for ocular research.

C R Munnerlyn, J R Gray, D R Hennings

    Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Neurons Associated With a Novel Motor Pattern Expressed During Metamorphosis of the Hawkmoth, Manduca sexta.

    The Biological bulletin·2017
    Same author

    Alignment requirements for mode matching in a confocal fabry-perot interferometer.

    Applied optics·2010
    Same author

    A simple laser interferometer.

    Applied optics·2010
    Same author

    Rough Surface Interferometry Using a CO(2) Laser Source.

    Applied optics·2010
    Same author

    Treatment of ulcerative colitis from the patient's perspective: a survey of preferences and satisfaction with therapy.

    Alimentary pharmacology & therapeutics·2009
    Same author

    Increased health costs from mandated Therapeutic Substitution of proton pump inhibitors in British Columbia.

    Alimentary pharmacology & therapeutics·2009

    This study introduces a new fluorophotometer for ocular research, optimizing optical parameters to reduce artifacts and improve signal detection for more accurate fluorometric studies.

    Area of Science:

    • Ophthalmology
    • Biomedical Optics
    • Analytical Chemistry

    Background:

    • Modified slit-lamp microscopes offer versatility but often lack optimization in key parameters like detector apertures and filters.
    • Improper control of optical parameters in fluorometric studies can lead to artifacts that obscure valuable data.
    • Existing instruments may require contact lenses, limiting their practical application in ocular research.

    Purpose of the Study:

    • To design and optimize a novel fluorophotometer for ocular research.
    • To enhance axial resolution and minimize detectable signals in ocular fluorometry.
    • To reduce artifacts and improve the signal-to-noise ratio for more reliable measurements.

    Main Methods:

    • Designed a new fluorophotometer with an optimized optical system, eliminating the need for a contact lens.

    Related Experiment Videos

  • Evaluated and compared standard barrier and excitation filters against photomultiplier sensitivity curves.
  • Employed double filters to minimize excitation light artifacts and a photon counting detection system for signal-to-noise optimization.

    • Main Results:

    • The new fluorophotometer design successfully optimized axial resolution and minimum detectable signal.
    • Utilizing double filters effectively reduced artifacts caused by unrejected excitation light.
    • The photon counting system achieved a dark current equivalent to 3 x 10(-10) g/ml, enabling a detection range down to 1 x 10(-10) g/ml.

    Conclusions:

    • The developed fluorophotometer offers improved performance for ocular research by minimizing artifacts and enhancing sensitivity.
    • Optimized optical parameters and detection systems are crucial for accurate and reliable fluorometric measurements in the eye.
    • This instrument provides a valuable tool for sensitive detection in ocular fluorometric studies.