Related Experiment Video

Updated: Jun 16, 2025

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence
06:52

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Published on: November 5, 2021

3.0K

Small protein ERSP encoded by LINC02870 promotes triple negative breast cancer progression via IRE1α/XBP1s activation

Xiaolu Wang1,2, Qianqian Wang1, Hong Wang1

  • 1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong, 510006, China.

Cell Death and Differentiation
|January 11, 2025
PubMed

Insights

Researchers discovered a new protein, ERSP, encoded by LINC01870, that drives triple-negative breast cancer (TNBC) progression by activating ER stress. Targeting ERSP may offer new therapeutic strategies for TNBC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Triple-negative breast cancer (TNBC) lacks targeted therapies, relying primarily on chemotherapy.
  • Long noncoding RNAs (lncRNAs) have emerging roles beyond non-coding functions, including encoding small proteins.
  • The role of lncRNA-encoded proteins in cancer progression is largely unexplored.

Purpose of the Study:

  • To identify and characterize novel lncRNA-encoded proteins involved in TNBC.
  • To investigate the function of a newly identified protein, ERSP, in TNBC pathogenesis.
  • To explore ERSP as a potential therapeutic target for TNBC.

Main Methods:

  • Identification and characterization of a novel small protein (ERSP) encoded by LINC01870.
  • Analysis of ERSP expression in TNBC tissues and correlation with patient prognosis.
  • In vitro and in vivo studies to assess the impact of ERSP loss on TNBC growth and metastasis.
  • Investigation of ERSP's mechanism of action involving the endoplasmic reticulum (ER) stress pathway, specifically the IRE1α/XBP1s branch.

Main Results:

  • A novel small protein, ERSP, encoded by LINC01870, was identified and localized to the ER.
  • ERSP expression was significantly elevated in TNBC tissues and correlated with poor patient prognosis.
  • Loss of ERSP function suppressed TNBC cell growth and metastasis in vitro and in vivo.
  • ERSP was found to activate the IRE1α/XBP1s pathway, enhancing the unfolded protein response (UPR) and promoting ER stress-related gene expression.

Conclusions:

  • The lncRNA-encoded protein ERSP plays a significant pro-oncogenic role in TNBC by modulating ER stress.
  • ERSP's activation of the IRE1α/XBP1s pathway is crucial for its oncogenic functions.
  • ERSP represents a promising novel therapeutic target for triple-negative breast cancer.

More Related Videos

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

6.9K
Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

6.8K

Related Concept Videos

Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K