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VAMP8 as a biomarker and potential therapeutic target for endothelial cell dysfunction in atherosclerosis

  • 0Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, 030032, China.
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January 12, 2025

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January 12, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Vesicle-associated membrane protein 8 (VAMP8) is upregulated in endothelial cell dysfunction associated with atherosclerosis. This study identifies VAMP8 as a potential pathogenic gene contributing to atherosclerosis development.

Area Of Science

  • Cardiovascular Biology
  • Molecular Medicine
  • Genetics

Background

  • Endothelial cell dysfunction is central to atherosclerosis pathophysiology.
  • Identifying key genes and pathways is crucial for understanding atherosclerosis.
  • This study investigates causal mechanisms in atherosclerotic endothelial dysfunction.

Purpose Of The Study

  • To uncover pivotal genes and pathways in endothelial cell dysfunction in atherosclerosis.
  • To ascertain causal effects and potential mechanisms of these genes.
  • To validate the role of identified genes, particularly VAMP8.

Main Methods

  • Collected and analyzed atherosclerosis datasets.
  • Constructed protein-protein interaction networks and molecular interaction maps.
  • Utilized Gene Ontology, KEGG, GSEA, SMR analysis, qPCR, and Western Blot.

Main Results

  • Identified 14 common-differentially expressed genes (co-DEGs).
  • Confirmed 10 potential causal genes via SMR analysis, including VAMP8.
  • VAMP8 was significantly upregulated in injured endothelial cells and patient blood samples.

Conclusions

  • Vesicle-associated membrane protein 8 (VAMP8) is a potential pathogenic gene in endothelial dysfunction.
  • VAMP8 upregulation is linked to atherosclerosis development.
  • Further research into VAMP8's role is warranted.

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