Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

464
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
464

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nstpbp5185, a novel benzimidazole derivative, suppresses PDGF signaling and reduces neointimal hyperplasia following vascular injury.

European journal of pharmacology·2026
Same author

Interleukin-1 receptor accessory protein expression could link to the insula-default mode network connection and clinical depression in fibromyalgia: a preliminary exploratory study.

Clinical and experimental rheumatology·2026
Same author

Harnessing engineered mesenchymal stem cell-derived extracellular vesicles for innovative cancer treatments.

Stem cell research & therapy·2025
Same author

Enhancing Hydroxyl Metabolite Analysis through In Situ Derivatization-Enabled Desorption Electrospray Ionization-Mass Spectrometry.

Analytical chemistry·2025
Same author

Integrated Glycosylation Analysis of Immunoglobulin Isotypes Reveals Expanded Humoral Remodeling in Elderly Tuberculosis Infection.

Molecular & cellular proteomics : MCP·2025
Same author

Choosing sweeteners wisely-nutrigenetic study on childhood obesity.

Nutrition & metabolism·2025

Related Experiment Video

Updated: Jun 3, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.0K

A sensitive post-column derivatization approach for enhancing hydroxyl metabolites detection.

Yen-Chu Lin1, Shiu-Wen Huang2, San-Yuan Wang3

  • 1Department of Pharmacy, College of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan.

Analytica Chimica Acta
|January 12, 2025
PubMed
Summary

A new post-column derivatization method using BBII reagent significantly enhances the detection of hydroxyl metabolites in liquid chromatography-mass spectrometry (LC-MS). This advance improves sensitivity for previously undetectable compounds in biological samples.

Keywords:
2-(4-boronobenzyl) isoquinolin-2-ium bromideHydroxyl metabolitesLC-MSPost-column derivatizationUntargeted metabolomics

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.2K

Related Experiment Videos

Last Updated: Jun 3, 2025

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
06:25

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

Published on: April 26, 2016

15.0K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

20.9K
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
08:06

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

9.2K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Metabolomics

Background:

  • Chemical derivatization is crucial for enhancing metabolite detection in LC-MS.
  • The reagent 2-(4-boronobenzyl) isoquinolin-2-ium bromide (BBII) improves ionization of hydroxyl metabolites.
  • BBII offers rapid reaction times, simplifying sample preparation.

Purpose of the Study:

  • To develop a novel post-column derivatization (PCD) method using BBII for improved LC-MS analysis of hydroxyl metabolites.
  • To enhance the detection sensitivity of a wide range of hydroxyl-containing compounds.

Main Methods:

  • Developed and optimized a post-column derivatization (PCD) method utilizing the BBII reagent.
  • Tested the method with 14 hydroxyl-containing compounds to assess sensitivity improvements.
  • Applied the BBII PCD method to analyze hydroxyl metabolites in glioblastoma cells treated with MFB.

Main Results:

  • The BBII PCD method successfully detected previously undetectable metabolites like glucose, ribose, and long-chain alcohols.
  • Sensitivity enhancements for hydroxyl metabolites ranged from 1.1 to 42.9-fold.
  • Analysis of glioblastoma cells revealed significantly reduced hydroxyl metabolite levels after MFB treatment.

Conclusions:

  • The novel BBII PCD method substantially improves hydroxyl metabolite detection sensitivity in LC-MS.
  • This technique is valuable for untargeted metabolomics in biological and clinical research.
  • It provides a robust tool for identifying metabolite changes in disease and therapeutic studies.