Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
  1. Home
  3. Carbon Dot Embedded Hybrid Microgel From Synthesis To Sensing: Experimental And Theoretical Approach.
  1. Home
  3. Carbon Dot Embedded Hybrid Microgel From Synthesis To Sensing: Experimental And Theoretical Approach.

Related Experiment Video

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

12.8K

Carbon dot embedded hybrid microgel from synthesis to sensing: Experimental and theoretical approach.

Neha Garg1, Ankita Garg1, Aman Bhalla1

  • 1Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India.

Analytica Chimica Acta
|January 12, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed a novel hybrid microgel using carbon quantum dots (CQDs) and poly-N isopropylacrylamide (PNIPAM) for highly sensitive and selective Tryptophan (Trp) detection. This advanced probe shows great potential for real-world applications in healthcare and environmental monitoring.

Keywords:
Carbon dotsPoly-N isopropylacrylamideSelectivitySensitivityTryptophan

More Related Videos

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.1K
Fabrication of 3D Carbon Microelectromechanical Systems C-MEMS
08:01

Fabrication of 3D Carbon Microelectromechanical Systems C-MEMS

Published on: June 17, 2017

12.2K

Related Experiment Videos

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

12.8K
Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.1K
Fabrication of 3D Carbon Microelectromechanical Systems C-MEMS
08:01

Fabrication of 3D Carbon Microelectromechanical Systems C-MEMS

Published on: June 17, 2017

12.2K

Area of Science:

  • Materials Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Advancements in artificial probes for amino acid analysis are rapidly growing.
  • Carbon quantum dots (CQDs) are increasingly utilized in material science.
  • Hybrid microgels incorporating CQDs remain an underexplored area.

Purpose of the Study:

  • To develop a highly fluorescent hybrid microgel for selective Tryptophan (Trp) sensing.
  • To create a sensitive bio-probe by integrating biocompatible CQDs with poly-N isopropylacrylamide (PNIPAM).
  • To optimize the sensing capabilities for accurate Trp quantification.

Main Methods:

  • In-situ synthesis of a hybrid microgel by combining CQDs and PNIPAM.
  • Surface passivation of CQDs with PNIPAM for controlled size and shape.
  • Optimization of sensing parameters including concentration, pH, and temperature.
  • Validation of selectivity in the presence of interfering biomolecules and real water samples.

    • Main Results:

    • Successfully prepared a fluorescent hybrid microgel for Trp sensing.
    • Achieved accurate and selective recognition of Trp.
    • Quantification of Trp over a wide linear range (10 μM–500 μM) with a low limit of detection (0.1 μM).

    Conclusions:

    • The integrated system demonstrates excellent selectivity for Trp sensing, even with similar biomolecules.
    • The sensor effectively detected Trp in various real water samples.
    • The hybrid material combines CQD biocompatibility and fluorescence with PNIPAM's thermo-responsive properties, offering potential for diagnostics, healthcare, and environmental remediation.