Related Experiment Video
Updated: Jun 2, 2025
07:55
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
Published on: April 11, 2018
14.2K
CIRCUS: CIRCUlating tumour cells in soft tissue Sarcoma - a short report.
Robin J Young1, Joanna E Chowdry2, Denis Cochonneau3
1Weston Park Cancer Centre, Sheffield Teaching Hospitals NHS Foundation Trust, Sheffield S10 2SJ, UK.
Cancer Drug Resistance (Alhambra, Calif.)
|January 13, 2025
Summary
Circulating tumour cells (CTCs) were successfully isolated from soft tissue sarcoma (STS) patients. CTCs showed heterogeneity and correlated with primary tumour size, suggesting potential prognostic value.
Area of Science:
- Oncology
- Cell Biology
- Medical Diagnostics
Background:
- Circulating tumour cells (CTCs) offer insights into tumour biology and drug resistance.
- Detecting CTCs in peripheral blood is crucial for understanding cancer progression.
- Soft tissue sarcoma (STS) patient CTCs require further characterization.
Purpose of the Study:
- To evaluate the isolation of CTCs from peripheral blood of STS patients.
- To assess the heterogeneity and clinical correlation of STS CTCs.
Main Methods:
- Combined microfluidic enrichment (Parosrtix™ PR1) and DEPArray™ sorting.
- Vimentin and cytokeratin staining for CTC identification.
- Analysis of circulating cell-free DNA (cfDNA) and correlation with clinical parameters.
Main Results:
- CTCs were isolated from 13 STS patients (7 localized, 6 metastatic).
- CTCs displayed significant heterogeneity in mesenchymal and epithelial marker expression.
- CTC numbers correlated with primary tumour size but not cfDNA levels.
Conclusions:
- CTCs are present in both localized and advanced STS patients.
- Further research is needed to characterize STS CTCs and their prognostic significance.
