Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Rare Case Reveals Important Consideration of the Diagnosis of Giant Cell Arteritis in Patients with Bilateral Painful Optic Perineuritis.

Reports (MDPI)·2026
Same author

The Impact of Hemoglobin Transfusion Thresholds in Moderate-to-Severe Blunt Traumatic Brain Injury on 6-Month Neurologic Outcomes: A Systematic Review and Meta-Analysis.

Neurology international·2026
Same author

Hitting A Nerve: T1 Nerve Root Injury Secondary to Open Heart Surgery.

The neurologist·2026
Same author

Fully Automated Production of Multiple <sup>11</sup>C-Labeled PET Tracers Using a Self-Cleaning TracerMaker Radiosynthesizer.

Journal of labelled compounds & radiopharmaceuticals·2026
Same author

Clinical application of artificial intelligence algorithms in detecting clival remodeling in the setting of pituitary neuroendocrine tumors/pituitary adenomas.

Frontiers in neurology·2026
Same author

Solitary thoracic Epstein-Barr virus-associated smooth muscle tumor presenting after kidney transplant: illustrative case.

Journal of neurosurgery. Case lessons·2026

Related Experiment Video

Updated: Jun 2, 2025

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
07:54

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Published on: October 25, 2011

18.6K

Evidence of Significant Intratumoral Transcriptomic Heterogeneity in Non-functioning Pituitary Adenomas Based on

Faraz Behzadi1, Parker L Woldt1, John T Tsiang1

  • 1Neurological Surgery, Loyola University Medical Center, Maywood, USA.

Cureus
|January 13, 2025
PubMed
Summary

This study reveals significant genetic differences within non-functioning pituitary adenomas (NFPA), highlighting variations in gene expression related to tumor location and texture. These findings offer potential new targets for NFPA gene therapy.

Keywords:
differential gene expressionpituitary adenomastranscriptomicstumor cell biologytumor texture

More Related Videos

Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.7K
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

7.9K

Related Experiment Videos

Last Updated: Jun 2, 2025

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
07:54

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Published on: October 25, 2011

18.6K
Comparative Lesions Analysis Through a Targeted Sequencing Approach
08:16

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

6.7K
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

7.9K

Area of Science:

  • Endocrinology
  • Genomics
  • Oncology

Background:

  • Non-functioning pituitary adenomas (NFPA) are typically treated with surgical resection.
  • Significant intratumoral heterogeneity exists in NFPA regarding growth rates and texture.
  • Understanding this heterogeneity is crucial for explaining tumor pathophysiology.

Purpose of the Study:

  • To identify variations in gene expression within different locations and textures of the same NFPA.
  • To correlate genetic heterogeneity with tumor characteristics.
  • To explore potential therapeutic targets based on genetic differences.

Main Methods:

  • NFPA tissue samples were collected from four adult patients undergoing endoscopic endonasal resection.
  • Next-generation transcriptomics was employed for gene expression analysis.
  • Significantly differentially expressed (SDE) genes were analyzed and categorized using gene ontology.

Main Results:

  • 164 SDE genes were identified, with 264 related to tumor hardness and 68 to location (core vs. edge).
  • Gene ontology analysis revealed annotations related to cell metabolism, cell-cell interactions, and cell division.
  • Tumor edges showed higher expression of cell-cell interaction genes (e.g., cadherin-binding proteins).
  • Soft tumor portions exhibited upregulated anaerobic metabolism and cell division genes.

Conclusions:

  • Significant intratumor genetic heterogeneity exists within NFPA, varying by location, texture, and patient.
  • Specific gene expression patterns correlate with tumor characteristics, such as edge location and soft texture.
  • These unique genetic profiles present opportunities for future biological function testing and potential gene therapy targets.