Jove
Visualize
Contact Us

Related Experiment Videos

Atomic absorption spectrophotometric determination of liver copper: collaborative study.

D L Osheim, P F Ross

    Journal - Association of Official Analytical Chemists
    |January 1, 1985
    PubMed
    Summary

    This study validated a new atomic absorption spectrophotometry method for determining copper in bovine liver. The validated method demonstrated reliable accuracy and precision, making it suitable for official food analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Disruption of sphingolipid metabolism and induction of equine leukoencephalomalacia by Fusarium proliferatum culture material containing fumonisin B(2) or B(3).

    Environmental toxicology and pharmacology·2011
    Same author

    An international collaborative study on a method for determination of formaldehyde in veterinary vaccines.

    Biologicals : journal of the International Association of Biological Standardization·2002
    Same author

    Fumonisin B1 promotes aflatoxin B1 and N-methyl-N'-nitro-nitrosoguanidine-initiated liver tumors in rainbow trout.

    Toxicology and applied pharmacology·2001
    Same author

    Serum copper concentrations in beef cows and heifers.

    Journal of the American Veterinary Medical Association·1999
    Same author

    Fusarium mycotoxins in corn and corn products in a high-risk area for gastric cancer in Shandong Province, China.

    Journal of AOAC International·1999
    Same author

    Screening nitrate in forages with a test strip: collaborative study.

    Journal of AOAC International·1999
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Analytical Chemistry
    • Food Science
    • Veterinary Medicine

    Background:

    • Accurate quantification of essential trace elements like copper in food matrices is crucial for both nutritional assessment and regulatory compliance.
    • Existing methods for copper determination in biological tissues may lack the necessary precision or sensitivity for comprehensive analysis.
    • The need for a standardized, reliable method for copper analysis in bovine liver was identified.

    Purpose of the Study:

    • To collaboratively validate a novel analytical method for determining copper concentrations in bovine liver samples.
    • To assess the method's accuracy, precision, and limit of detection across multiple independent laboratories.
    • To establish a robust and officially recognized procedure for copper analysis in bovine liver.

    Main Methods:

    Related Experiment Videos

    • Collaborative study involving eleven laboratories analyzing blind duplicate bovine liver samples.
    • Samples were either naturally copper-rich or spiked to cover a range of copper levels.
    • Method involved acid digestion (concentrated HNO3), dilution, and analysis via atomic absorption spectrophotometry (AAS).

    Main Results:

    • Intralaboratory coefficients of variation (CVo) ranged from 5.6% to 19%.
    • Interlaboratory coefficients of variation (CVx) ranged from 7.1% to 21%.
    • The method's lower limit of detection was determined to be approximately 1 mg copper/kg tissue.

    Conclusions:

    • The validated atomic absorption spectrophotometry method provides reliable and reproducible results for copper determination in bovine liver.
    • The method's performance across multiple laboratories supports its suitability for routine analysis.
    • The method has been officially adopted for first action use, indicating its acceptance for regulatory purposes.