Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic transfer in Halobacterium volcanii.

M Mevarech, R Werczberger

    Journal of Bacteriology
    |April 1, 1985
    PubMed
    Summary

    Researchers demonstrated a native genetic transfer system in Halobacterium volcanii, an archaeon. This system was shown using auxotrophic mutants created through chemical mutagenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Halophilic enzymes: proteins with a grain of salt.

    Biophysical chemistry·2000
    Same author

    Evidence for post-translational membrane insertion of the integral membrane protein bacterioopsin expressed in the heterologous halophilic archaeon Haloferax volcanii.

    The Journal of biological chemistry·2000
    Same author

    The extremely halophilic archaeon Haloferax volcanii has two very different dihydrofolate reductases.

    Molecular microbiology·2000
    Same author

    Insights into the molecular relationships between malate and lactate dehydrogenases: structural and biochemical properties of monomeric and dimeric intermediates of a mutant of tetrameric L-[LDH-like] malate dehydrogenase from the halophilic archaeon Haloarcula marismortui.

    Biochemistry·2000
    Same author

    Overexpression Beadex mutations and loss-of-function heldup-a mutations in Drosophila affect the 3' regulatory and coding components, respectively, of the Dlmo gene.

    Genetics·1998
    Same author

    Structural features of halophilicity derived from the crystal structure of dihydrofolate reductase from the Dead Sea halophilic archaeon, Haloferax volcanii.

    Structure (London, England : 1993)·1998

    Area of Science:

    • Microbiology
    • Genetics
    • Archaeal biology

    Background:

    • Halobacterium volcanii is an extremely halophilic archaeon.
    • Understanding genetic transfer mechanisms in archaea is crucial for molecular biology.
    • Previous studies have not fully elucidated native genetic transfer in H. volcanii.

    Purpose of the Study:

    • To demonstrate the existence of a native genetic transfer system in Halobacterium volcanii.
    • To characterize the mechanism of genetic exchange in this archaeal species.

    Main Methods:

    • Generation of auxotrophic mutants of Halobacterium volcanii via chemical mutagenesis.
    • Experimental setup to observe and quantify genetic transfer between mutant strains.

    Main Results:

    • Successful demonstration of genetic transfer between auxotrophic mutants of H. volcanii.
    • Evidence supporting a functional, native system for DNA exchange within the organism.

    Conclusions:

    • Halobacterium volcanii possesses an intrinsic system for genetic transfer.
    • This finding opens new avenues for genetic manipulation and research in extremophilic archaea.

    Related Experiment Videos