Genome-Wide Association Study and Genomic Predictions for Hydroxycinnamate Concentrations in Maize Stover

Hydroxycinnamates, like ferulate (FA) and p-coumarate (pCA), are important components of maize cell walls, which influence pest resistance, ruminal digestibility, and biofuel production. Increasing their concentration has been linked to increased pest resistance, but also may lead to a decrease in nutritional value or bioethanol production efficiency. Therefore, improving forage quality or biofuel production without compromising plant resistance and a thorough understanding of the biosynthesis and deposition of these compounds is necessary, especially in stover, which is the feedstock for second-generation biofuel production and determines animal forage quality. This study aimed to identify genomic regions associated with hydroxycinnamates and to develop genomic prediction models to determine the best selection approach to modify hydroxycinnamate content. Although heritability estimates for hydroxycinnamates were moderate, direct phenotypic selection is discouraged because hydroxycinnamate quantification is laborious and time-consuming. Negative genotypic correlations were observed between animal digestibility and pCA content and positive with diferulates content, suggesting differing effects compared to previous studies on maize pith. However, no colocalizations with digestibility QTLs were found, highlighting the need for further research. Given the moderate predictive capacity of GBLUP prediction models, genotypic selection is proposed as the most promising alternative for modifying hydroxycinnamate content.