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Updated: Jun 2, 2025
Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells
Published on: December 16, 2021
Protocol for isolating and purifying murine liver sinusoidal endothelial cells for in vitro culture and functional
Sophia Papaioannou1, Jia-Xiang See1, Tinja Baljkas2
1Department of Immunobiochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany; European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
Abstract:
Liver sinusoidal endothelial cells (LSECs) line the liver sinusoids and play a crucial role in liver function. Isolating LSECs is beneficial for their functional evaluation in vitro. Here, we provide a protocol for obtaining purified LSECs from mice via gradient centrifugation and magnetic cell sorting (MACS), yielding cells suitable for culture and downstream analyses. We describe steps for culturing the purified LSECs and demonstrate their evaluation by flow cytometry. For complete details on the use and execution of this protocol, please refer to Papaioannou et al.1.
