Preparation, Modification, Quantitation, and Dentin Biomodification Activity of Selectively Enriched...

Area of Science:

• Analytical Chemistry
• Natural Products Chemistry
• Biomaterials Science

Background:

• Current proanthocyanidin (PAC) quantification methods lack accuracy and specificity.
• Existing assays require extensive sample preparation and lack reliable reference standards.
• There is a need for precise analytical methods to support the development of plant-sourced biomaterials for dental applications.

Purpose of the Study:

• To develop and validate a quantitative NMR (qNMR) method for accurate PAC content determination.
• To characterize PAC DESIGNER materials using advanced chromatographic and spectroscopic techniques.
• To establish a robust quality control methodology for PAC-based biomaterials.

Main Methods:

• Characterization of 12 PAC DESIGNER materials using centrifugal partition chromatography (CPC) and size-exclusion chromatography (SEC).
• Synthesis of A-type PAC DESIGNERs from AB-type precursors using DPPH and TEMPO radicals.
• Development of a qNMR method with a non-PAC internal calibrant, combined with diol-HPLC for total PAC quantification.

Main Results:

• The qNMR method accurately quantified total PAC content in DESIGNER materials, ranging from 67.5% to 96.9%.
• Complementarity of diol-HPLC and qNMR was demonstrated for assessing PAC concentration and stability.
• Successful generation of A-type PAC DESIGNERs was achieved.

Conclusions:

• The developed qNMR method provides accurate and specific quantification of PACs.
• This methodology enables the generation of standardized PAC materials and rigorous quality control.
• The findings support the advancement of PAC-based biomaterials for dental (pre)clinical studies.